Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- PA5-11946 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-SMURF2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- Lot Dependent
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Hippocampal neurons using a SMURF2 polyclonal antibody (Product # PA5-11946). Hippocampal neurons were fixed at stage 3, stained with anti-SMURF2 polyclonal antibody (Product # PA5-11946) (red) and anti-Kinesin-2 (green) antibodies, and analyzed by confocal microscopy.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of hippocampal neurons, transfected 2 hr after plating, with expression vectors for EGFP, EGFP-tagged Par3-4N/2, Par3-PDZ2, Par3-PDZ3, Smurf2-HECT (HECT), Smurf2-HECT-C716A (HECT CA), and shRNA directed against mPar3 (Par3 RNAi), or vectors for the anti-Par3 shRNA and human Myc-Par3 (RNAi + h Par3) (green) using a SMURF2 polyclonal antibody (Product # PA5-11946) (red). Axons are marked by arrowheads. The marked growth cones are shown at a higher magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of formalin-fixed, paraffin-embedded human cancer tissue using a SMURF2 polyclonal antibody (Product # PA5-11946), followed by HRP-conjugated secondary antibody and DAB staining.