Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX105655 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX105655, RRID:AB_2037103
- Product name
- GSTO1 antibody [N1C3]
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references Identification of glutathione S-transferase omega 1 (GSTO1) protein as a novel tumor-associated antigen and its autoantibody in human esophageal squamous cell carcinoma.
Li Y, Zhang Q, Peng B, Shao Q, Qian W, Zhang JY
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2014 Nov;35(11):10871-7
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2014 Nov;35(11):10871-7
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: HepG2 (GTX27900) 12% SDS PAGE GTX105655 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Mouse tissue extract (50 ?g) was separated by 12% SDS-PAGE, and the membrane was blotted with GSTO1 antibody [N1C3] (GTX105655) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and GSTO1 knockout (KO) 293T cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with GSTO1 antibody [N1C3] (GTX105655) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded GSTA2 xenograft, using GSTO1(GTX105655) antibody at 1:100 dilution.