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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-31185 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GST Omega 1 140A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, HepG2, mouse colon, rat liver.
- Concentration
- 1 mg/mL
Submitted references Glutathione transferase Omega 1-1 (GSTO1-1) modulates Akt and MEK1/2 signaling in human neuroblastoma cell SH-SY5Y.
Saisawang C, Wongsantichon J, Robinson RC, Ketterman AJ
Proteins 2019 Jul;87(7):588-595
Proteins 2019 Jul;87(7):588-595
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of GST Omega 1 140A was performed by separating 50 µg of mouse tissue extract by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a GST Omega 1 140A Polyclonal Antibody (Product # PA5-31185) at a dilution of 1:5000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of GST Omega 1 140A was performed by separating 50 µg of rat tissue extract by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a GST Omega 1 140A Polyclonal Antibody (Product # PA5-31185) at a dilution of 1:5,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using GST Omega 1 140A Polyclonal Antibody (Product # PA5-31185). Sample (30 µg of whole cell lysate). Lane A: HepG2. 12% SDS PAGE. GST Omega 1 140A Polyclonal Antibody (Product # PA5-31185) diluted at 1:5,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- GST Omega 1 140A Polyclonal Antibody detects GSTO1 protein at cytoplasm on human lung carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung carcinoma. GST Omega 1 140A Polyclonal Antibody (Product # PA5-31185) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Coimmunoprecipitation of p-Akt or p-MEK1/2 and human GSTO1-1. The cell lysates were employed for immunoprecipitation (IP) and immunoblotting (IB). IP was performed using anti-phospho-Akt, anti-phospho-MEK1/2 antibody or anti-human GSTO1 antibody. IB with anti-human GSTO1, anti-phospho-Akt, or anti-phospho-MEK1/2 antibody was then performed on membranes to which the cell proteins had been transferred. The membranes were then stripped and re-probed with the respective IP antibody to show loading. Con. is nontreated control cells, lipopolysaccharide (LPS) cells were treated with 1 mug/mL LPS, and ML175 cells were treated with 1 muM ML175 for 10 minutes
