Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [6]
- Immunohistochemistry [4]
- Flow cytometry [2]
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- Product number
- MA5-42456 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Argonaute 3 Recombinant Rabbit Monoclonal Antibody (JJ201-07)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: N2A, SHG-44, F9, NCCIT, rat testis tissue, rat spinal cord tissue, mouse brain tissue, rat brain tissue. Subcellular Location: Cytoplasm,Nucleus.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- JJ201-07
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of EIF2C3 in N2A cells (green) using Argonaute 3 Recombinant Monoclonal Antibody (Product # MA5-42456). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of EIF2C3 in SHG-44 cells (green) using Argonaute 3 Recombinant Monoclonal Antibody (Product # MA5-42456). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of EIF2C3 in NCCIT cells (green) using Argonaute 3 Recombinant Monoclonal Antibody (Product # MA5-42456). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of EIF2C3 in SHG-44 cells (green) using Argonaute 3 Recombinant Monoclonal Antibody (Product # MA5-42456). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of EIF2C3 in F9 cells (green) using Argonaute 3 Recombinant Monoclonal Antibody (Product # MA5-42456). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of EIF2C3 in NCCIT cells (green) using Argonaute 3 Recombinant Monoclonal Antibody (Product # MA5-42456). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of paraffin-embedded rat testis tissue using Argonaute 3 Recombinant Monoclonal Antibody (Product # MA5-42456). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of paraffin-embedded rat spinal cord tissue using Argonaute 3 Recombinant Monoclonal Antibody (Product # MA5-42456). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of paraffin-embedded rat brain tissue using Argonaute 3 Recombinant Monoclonal Antibody (Product # MA5-42456). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of paraffin-embedded mouse brain tissue using Argonaute 3 Recombinant Monoclonal Antibody (Product # MA5-42456). Counter stained with hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of Argonaute 3 in N2A cells using Argonaute 3 Recombinant Monoclonal Antibody (Product # MA5-42456) at 1:50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of Argonaute 3 in N2A cells using Argonaute 3 Recombinant Monoclonal Antibody (Product # MA5-42456) at 1:50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.