AP5840c

antibody from Abcepta

Targeting: GSTO2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP5840c

Provider

Abcepta

Proper citation

Abgent Cat#AP5840c, RRID:AB_10818108

Product name

GSTO2 Antibody (Center)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

0.5 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

GSTO2 Antibody (Center) (Cat. #AP5840c) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the GSTO2 antibody detected the GSTO2 protein (arrow).

Primary Ab dilution

1:1000

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

Western blot analysis of GSTO2 (arrow) using rabbit polyclonal GSTO2 Antibody (Center) (Cat. #AP5840c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GSTO2 gene.

Primary Ab dilution

1:1000

Immunocytochemistry

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

Confocal immunofluorescent analysis of GSTO2 Antibody (Center)(Cat#AP5840c) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Primary Ab dilution

1:10~50

Flow cytometry

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

GSTO2 Antibody (Center) (Cat. #AP5840c) flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Primary Ab dilution

1:10~50