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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
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- Product number
- PA5-27123 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BCL7A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: HeLaS3, Neuro2A, GL261. Predicted reactivity: Mouse (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using BCL7A Polyclonal Antibody (Product # PA5-27123). Sample (30 µg of whole cell lysate). A: HeLa S3.15% SDS PAGE. BCL7A Polyclonal Antibody (Product # PA5-27123) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- BCL7A Polyclonal Antibody detects BCL7A protein by Western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract. B. 30 µg GL261 whole cell lysate/extract.12 % SDS-PAGE. BCL7A Polyclonal Antibody (Product # PA5-27123) dilution: 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- BCL7A Polyclonal Antibody detects BCL7A protein at nucleus by immunofluorescent analysis. Sample: SKNSH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BCL7A protein stained by BCL7A Polyclonal Antibody (Product # PA5-27123) diluted at 1:1,000. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- BCL7A Polyclonal Antibody detects BCL7A protein at nucleus on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. BCL7A Polyclonal Antibody (Product # PA5-27123) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- BCL7A Polyclonal Antibody detects BCL7A protein at nucleus on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. BCL7A Polyclonal Antibody (Product # PA5-27123) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- BCL7A Polyclonal Antibody detects BCL7A protein at nucleus on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. BCL7A Polyclonal Antibody (Product # PA5-27123) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded oral CA cell lines Ca922 xenograft, using BCL7A (Product # PA5-27123) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
