Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [2]
- Immunohistochemistry [5]
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Validation data
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- Product number
- GTX111525 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX111525, RRID:AB_10724113
- Product name
- Hexokinase II antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Adenine Nucleotide Translocase 2 as an Enzyme Related to [18F] FDG Accumulation in Various Cancers.
PIM2-mediated phosphorylation of hexokinase 2 is critical for tumor growth and paclitaxel resistance in breast cancer.
Proteomic analysis of oral cancer reveals new potential therapeutic targets involved in the Warburg effect.
Lee CH, Kim MJ, Lee HH, Paeng JC, Park YJ, Oh SW, Chai YJ, Kim YA, Cheon GJ, Kang KW, Youn H, Chung JK
Molecular imaging and biology : MIB : the official publication of the Academy of Molecular Imaging 2018 Sep 17;
Molecular imaging and biology : MIB : the official publication of the Academy of Molecular Imaging 2018 Sep 17;
PIM2-mediated phosphorylation of hexokinase 2 is critical for tumor growth and paclitaxel resistance in breast cancer.
Yang T, Ren C, Qiao P, Han X, Wang L, Lv S, Sun Y, Liu Z, Du Y, Yu Z
Oncogene 2018 Nov;37(45):5997-6009
Oncogene 2018 Nov;37(45):5997-6009
Proteomic analysis of oral cancer reveals new potential therapeutic targets involved in the Warburg effect.
Huang YP, Chang NW
Clinical and experimental pharmacology & physiology 2017 Aug;44(8):880-887
Clinical and experimental pharmacology & physiology 2017 Aug;44(8):880-887
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Supportive validation
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- Experimental details
- Sample (30 ?g of whole cell lysate) A: IMR32 7.5% SDS PAGE GTX111525 diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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- GeneTex (provider)
- Main image
- Experimental details
- Sample (20 ?g of whole cell lysate) A: mouse brain 7.5% SDS PAGE GTX111525 diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hexokinase II antibody (GTX111525) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hexokinase II antibody (GTX111525) diluted at 1:10000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Hexokinase II antibody detects HK2 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37¢J for 30 min.Green: HK2 protein stained by Hexokinase II antibody (GTX111525) diluted at 1:500.Blue: Hoechst 33342 staining.
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- Experimental details
- Hexokinase II antibody detects Hexokinase II protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: Hexokinase II protein stained by Hexokinase II antibody (GTX111525) diluted at 1:500.Blue: Hoechst 33342 staining.
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Hexokinase II antibody detects HK2 protein at cytosol on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. Hexokinase II antibody (GTX111525) dilution: 1:500.
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- GeneTex (provider)
- Main image
- Experimental details
- Hexokinase II antibody detects Hexokinase II protein at cytosol on mouse muscle by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. Hexokinase II antibody (GTX111525) dilution: 1:500.
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- GeneTex (provider)
- Main image
- Experimental details
- Hexokinase II antibody detects Hexokinase II protein at cytoplasm in rat kidney by immunohistochemical analysis. Sample: Paraffin-embedded rat kidney. Hexokinase II antibody (GTX111525) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Hexokinase II antibody detects Hexokinase II protein at cytoplasm in rat heart by immunohistochemical analysis. Sample: Paraffin-embedded rat heart. Hexokinase II antibody (GTX111525) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Hexokinase II antibody detects Hexokinase II protein at cytoplasm in rat stomach by immunohistochemical analysis. Sample: Paraffin-embedded rat stomach. Hexokinase II antibody (GTX111525) diluted at 1:500.