R32605

antibody from NSJ Bioreagents

Targeting: HK2

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

R32605

Provider

NSJ Bioreagents

Product name

Hexokinase II Antibody

Antibody type

Polyclonal

Description

This highly specific Hexokinase II antibody is suitable for use in Western blot applications with human samples.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Vial size

100 ug

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Storage

After reconstitution, the Hexokinase II antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) rat testis, 2) mouse testis and 3) human Jurkat lysate with Hexokinase II antibody at 0.5ug/ml. Predicted molecular weight ~102 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) human HeLa, 2) human MCF7, 3) human HepG2, 4) rat skeletal muscle, 5) rat heart, 6) mouse skeletal muscle and 7) mouse heart tissue lysate with Hexokinase II antibody at 0.5ug/ml. Predicted molecular weight ~102 kDa.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human breast cancer tissue with Hexokinase II antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE mouse skeletal muscle tissue with Hexokinase II antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE rat skeletal muscle tissue with Hexokinase II antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of fixed and permeabilized human HepG2 cells with Hexokinase II antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Hexokinase II antibody.