AF7085

antibody from R&D Systems

Targeting: CRX CORD2, CRD, LCA7, OTX3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF7085

Provider

R&D Systems

Product name

Human CRX Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human CRX in direct ELISAs and Western blots.

Reactivity

Human

Host

Sheep

Conjugate

Unconjugated

Antigen sequence

O43186


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Patient-specific induced pluripotent stem cells to evaluate the pathophysiology of TRNT1-associated Retinitis pigmentosa.

Sharma TP, Wiley LA, Whitmore SS, Anfinson KR, Cranston CM, Oppedal DJ, Daggett HT, Mullins RF, Tucker BA, Stone EM
Stem cell research 2017 May;21:58-70

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cGMP production of patient-specific iPSCs and photoreceptor precursor cells to treat retinal degenerative blindness.

Wiley LA, Burnight ER, DeLuca AP, Anfinson KR, Cranston CM, Kaalberg EE, Penticoff JA, Affatigato LM, Mullins RF, Stone EM, Tucker BA
Scientific reports 2016 Jul 29;6:30742

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human CRX by Western Blot. Western blot shows lysates of Y-79 human retinoblastoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human CRX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7085) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CRX at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

CRX in Y-79 Human Cell Line. CRX was detected in immersion fixed Y-79 human retinoblastoma cell line using Sheep Anti-Human CRX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7085) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.