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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Immunocytochemistry [1]
- Other assay [2]
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- Product number
- PA5-31448 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PIWIL4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: PIWIL4-transfected 293T. Predicted reactivity: Rat (80%), Pig (85%), Rhesus Monkey (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.69 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Possible role of HIWI2 in modulating tight junction proteins in retinal pigment epithelial cells through Akt signaling pathway.
Expression and Regulation of PIWIL-Proteins and PIWI-Interacting RNAs in Rheumatoid Arthritis.
Sivagurunathan S, Palanisamy K, Arunachalam JP, Chidambaram S
Molecular and cellular biochemistry 2017 Mar;427(1-2):145-156
Molecular and cellular biochemistry 2017 Mar;427(1-2):145-156
Expression and Regulation of PIWIL-Proteins and PIWI-Interacting RNAs in Rheumatoid Arthritis.
Pleštilová L, Neidhart M, Russo G, Frank-Bertoncelj M, Ospelt C, Ciurea A, Kolling C, Gay RE, Michel BA, Vencovský J, Gay S, Jüngel A
PloS one 2016;11(11):e0166920
PloS one 2016;11(11):e0166920
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of PIWIL4 in paraformaldehyde-fixed MCF-7 cells using a PIWIL4 polyclonal antibody (Product # PA5-31448) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig 1 Expression of PIWIL genes A1. mRNA expression of PIWIL2 and PIWIL4 in synovial tissues (ST) from RA and OA patients. Mean dCt PIWIL2 in RAST 5.3 and in OAST 5.5; mean dCt PIWIL4 in RAST 3.6 and in OAST 2.9. PIWIL1 and PIWIL3 were not expressed. A2. mRNA expression of PIWIL2 and PIWIL4 in isolated synovial fibroblasts (SF) from RA and OA patients. Mean dCt PIWIL2 in RASF 2.2 and in OASF 1.3; mean dCt PIWIL4 in RASF 1.3 and in OASF 1.2. PIWIL1 and PIWIL3 were not expressed. B. PIWIL4 protein was detected by Western blot in both RASF and OASF in the nucleus, but not in the cytoplasm. PIWIL2 expression was weak. C. Immunofluorescence has shown the presence of PIWIL4 protein predominantly in the cell nucleus and in the perinuclear regions (representative example of a RASF culture).
