Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00005162-M03 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00005162-M03, RRID:AB_547487
- Product name
- Mouse Monoclonal Pyruvate Dehydrogenase E1 beta subunit Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. PDHB - pyruvate dehydrogenase (lipoamide) beta
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Proteomics of uveal melanomas suggests HSP-27 as a possible surrogate marker of chromosome 3 loss.
Coupland SE, Vorum H, Mandal N, Kalirai H, Honoré B, Urbak SF, Lake SL, Dopierala J, Damato B
Investigative ophthalmology & visual science 2010 Jan;51(1):12-20
Investigative ophthalmology & visual science 2010 Jan;51(1):12-20
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Pyruvate Dehydrogenase E1 beta subunit Antibody (2B2) [H00005162-M03] - Analysis of PDHB expression in transfected 293T cell line by PDHB monoclonal antibody (M03), clone 2B2. Lane 1: PDHB transfected lysatE (39.2 KDa). Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Pyruvate Dehydrogenase E1 beta subunit Antibody (2B2) [H00005162-M03] - Analysis of PDHB expression in HeLa (Cat # L013V1).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Pyruvate Dehydrogenase E1 beta subunit Antibody (2B2) [H00005162-M03] - Detection limit for recombinant GST tagged PDHB is approximately 1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1 beta subunit Antibody (2B2) [H00005162-M03] - Analysis of monoclonal antibody to PDHB on formalin-fixed paraffin-embedded human heart. Antibody concentration 3 ug/ml