Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [5]
- Flow cytometry [1]
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Validation data
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- Product number
- MA5-32757 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PDHB Recombinant Rabbit Monoclonal Antibody (JM66-33)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- JM66-33
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PDHB in mouse kidney tissue (1) and PC-12 (2) cell lysate using a PDHB Monoclonal antibody (Product # MA5-32757) at a dilution of 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PDHB in HepG2 (1) and Hela (2) cell lysate using a PDHB Monoclonal antibody (Product # MA5-32757) at a dilution of 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of PDHB of paraffin-embedded rat heart tissue using a PDHB Monoclonal antibody (Product #MA5-32757). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of PDHB of paraffin-embedded Human stomach cancer tissue using a PDHB Monoclonal antibody (Product #MA5-32757). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of PDHB of paraffin-embedded Human kidney tissue using a PDHB Monoclonal antibody (Product #MA5-32757). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of PDHB of paraffin-embedded Mouse brain tissue using a PDHB Monoclonal antibody (Product #MA5-32757). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of PDHB of paraffin-embedded Mouse hippocampus tissue using a PDHB Monoclonal antibody (Product #MA5-32757). Counter stained with hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometric analysis of PDHB in Hela cells using a PDHB Monoclonal Antibody (Product # MA5-32757) at a dilution of 1:100, as seen in red compared with an unlabelled control (cells without incubation with primary antibody; black).