14744-1-AP

antibody from Invitrogen Antibodies

Targeting: PDHB PDHE1B

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

14744-1-AP

Provider

Invitrogen Antibodies

Product name

PDHB Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MAAVSGLVR RPLREVSGLL KRRFHWTAPA ALQVTVRDAI NQGMDEELER DEKVFLLGEE VAQYDGAYKV SRGLWKKYGD KRIIDTPISE MGFAGIAVGA AMAGLRPICE FMTFNFSMQA IDQVINSAAK TYYMSGGLQP VPIVFRGPNG ASAGVAAQHS QCFAAWYGHC PGLKVVSPWN SEDAKGLIKS AIRDNNPVVV LENELMYGVP FEFPPEAQSK DFLIPIGKAK IERQGTHITV VSHSRPVGHC LEAAAVLSKE GVECEVINMR TIRPMDMETI EASVMKTNHL VTVEGGWPQF GVGAEICARI MEGPAFNFLD APAVRVTGAD VPMPYAKILE DNSIPQVKDI IFAIKKTLNI (1-359 aa encoded by BC000439)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.27 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14744-1-AP (PDHB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human heart tissue were subjected to SDS PAGE followed by western blot with 14744-1-AP (PDHB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Various lysates were subjected to SDS PAGE followed by western blot with 14744-1-AP (PDHB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

K-562 cells were subjected to SDS PAGE followed by western blot with 14744-1-AP (PDHB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human skin cancer using 14744-1-AP (PDHB antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human skin cancer using 14744-1-AP (PDHB antibody) at dilution of 1:100 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using (Product # 14744-1-AP) (PDHB antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-PDHB (IP:14744-1-AP, 4ug; Detection:14744-1-AP 1:300) with mouse heart tissue lysate 4000ug.