Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
Reference
Comment
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- Product number
- H00005238-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00005238-M01, RRID:AB_425591
- Product name
- PGM3 monoclonal antibody (M01), clone 1E2-1B12
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant PGM3.
- Antigen sequence
MDLGAITKYSALHAKPNGLILQYGTAGFRTKAEHL
DHVMFRMGLLAVLRSKQTKSTIGVMVTASHNPEED
NGVKLVDPLGEMLAPSWEEHATCLANAEEQDMQRV
LIDISEKEAVNLQQDAFVVIGRDTRPSSEKLSQSV
IDGVTVLGGQFHDYGLLTTPQLHYMVYCRNTGGRY
GKATIEGYYQKLSKAFVELTKQASCSGDEYRSLKV
DCANGIGALKLREMEHYFSQGLSVQLFNDGSKGKL
NHLCGADFVKSHQKPPQGMEIKSNERCCSFDGDAD
RIVYYYHDADGHFHLIDGDKIATLISSFLKELLVE
IGESLNIGVVQTAYANGSSTRYLEEVMKVPVYCTK
TGVKHLHHKAQEFDIGVYFEANGHGTALFSTAVEM
KIKQSAEQLEDKKRKAAKMLENIIDLFNQAAGDAI
SDMLVIEAILALKGLTVQQWDALYTDLPNRQLKVQ
VADRRVISTTNAERQAVTPPGLQEAINDLVKKYKL
SRAFVRPSGTEDVVRVYAEADSQESADHLAHEVSL
AVFQLAGGIGERPQPGF- Isotype
- IgG
- Antibody clone number
- 1E2-1B12
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- PGM3 monoclonal antibody (M01), clone 1E2-1B12 Western Blot analysis of PGM3 expression in HeLa ( Cat # L013V1 ).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of PGM3 expression in transfected 293T cell line by PGM3 monoclonal antibody (M01), clone 1E2-1B12.Lane 1: PGM3 transfected lysate(59.9 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged PGM3 is approximately 3ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of PGM3 transfected lysate using anti-PGM3 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with PGM3 MaxPab rabbit polyclonal antibody.
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to PGM3 on formalin-fixed paraffin-embedded human colon tissue. [antibody concentration 2 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol