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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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- Product number
- PA5-55058 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SMARCC1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: LLTERQNFHM EQLKYAELRA RQQMEQQQHG QNPQQAHQHS GGPGLAPLGA AGHPGMMPHQ QPPPYPLMHH QMPPPHPPQP GQIPGPGSMM PGQHMPGRMI PTVAANIHPS GSGPTPPGMP PMPGNILGPR VPLTAPNGMY P Highest antigen sequence identity to the following orthologs: Mouse - 94%, Rat - 95%.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SMARCC1 in Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells). Samples were probed using a SMARCC1 Polyclonal Antibody (Product # PA5-55058).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SMARCC1 in Lane 1: Marker (kDa) 230, 130, 95, 72, 56, 36, 28, 17, 11; Lane 2: Human cell line RT-4. Samples were probed using a SMARCC1 Polyclonal Antibody (Product # PA5-55058).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of SMARCC1 in human cell line U-2 OS shows positivity in nucleus but excluded from the nucleoli. Samples were probed using a SMARCC1 Polyclonal Antibody (Product # PA5-55058).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SMARCC1 in human testis and skeletal muscle tissues using SMARCC1 Polyclonal Antibody (Product # PA5-55058). Corresponding SMARCC1 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SMARCC1 in human testis using SMARCC1 Polyclonal Antibody (Product # PA5-55058) shows high expression.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SMARCC1 in human skeletal muscle using SMARCC1 Polyclonal Antibody (Product # PA5-55058) shows low expression as expected.
