Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunohistochemistry [5]
- Other assay [1]
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- Product number
- PA5-80195 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TSPAN12 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL. Positive Control - WB: human Hela cell, human SW620 cell, rat kidney tissue, rat brain tissue, mouse brain tissue. IHC: human lung cancer tissue, mouse lung tissue, rat lung tissue, human placenta tissue, human rectal cancer tissue.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 500 µg/mL
- Storage
- -20°C
Submitted references Ring finger protein 152-dependent degradation of TSPAN12 suppresses hepatocellular carcinoma progression.
Wan J, Liu S, Sun W, Yu H, Tang W, Liu W, Ji J, Liu B
Cancer cell international 2021 Feb 18;21(1):122
Cancer cell international 2021 Feb 18;21(1):122
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of TSPAN12 on paraffin-embedded human placenta tissue. Antigen retrieval was performed using citrate buffer (pH6, epitope retrieval solution) for 20 mins. Sample was blocked using 10% goat serum, incubated with TSPAN12 polyclonal antibody (Product# PA5-80195) with a dilution of 1 µg/mL (overnight at 4°C), and followed by biotinylated goat anti-rabbit IgG (30 minutes at 37°C). Development was performed using Streptavidin-Biotin-Complex (SABC) with DAB chromogen method.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of TSPAN12 on paraffin-embedded human rectal cancer tissue. Antigen retrieval was performed using citrate buffer (pH6, epitope retrieval solution) for 20 mins. Sample was blocked using 10% goat serum, incubated with TSPAN12 polyclonal antibody (Product# PA5-80195) with a dilution of 1 µg/mL (overnight at 4°C), and followed by biotinylated goat anti-rabbit IgG (30 minutes at 37°C). Development was performed using Streptavidin-Biotin-Complex (SABC) with DAB chromogen method.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of TSPAN12 on paraffin-embedded human lung cancer tissue. Antigen retrieval was performed using citrate buffer (pH6, epitope retrieval solution) for 20 mins. Sample was blocked using 10% goat serum, incubated with TSPAN12 polyclonal antibody (Product# PA5-80195) with a dilution of 1 µg/mL (overnight at 4°C), and followed by biotinylated goat anti-rabbit IgG (30 minutes at 37°C). Development was performed using Streptavidin-Biotin-Complex (SABC) with DAB chromogen method.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of TSPAN12 on paraffin-embedded rat lung tissue. Antigen retrieval was performed using citrate buffer (pH6, epitope retrieval solution) for 20 mins. Sample was blocked using 10% goat serum, incubated with TSPAN12 polyclonal antibody (Product# PA5-80195) with a dilution of 1 µg/mL (overnight at 4°C), and followed by biotinylated goat anti-rabbit IgG (30 minutes at 37°C). Development was performed using Streptavidin-Biotin-Complex (SABC) with DAB chromogen method.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of TSPAN12 on paraffin-embedded mouse lung tissue. Antigen retrieval was performed using citrate buffer (pH6, epitope retrieval solution) for 20 mins. Sample was blocked using 10% goat serum, incubated with TSPAN12 polyclonal antibody (Product# PA5-80195) with a dilution of 1 µg/mL (overnight at 4°C), and followed by biotinylated goat anti-rabbit IgG (30 minutes at 37°C). Development was performed using Streptavidin-Biotin-Complex (SABC) with DAB chromogen method.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 4 TSPAN12 was associated with RNF152. a The interaction protein network of RNF152 revealed by the BioGRID database ( https://thebiogrid.org/ ). b The cell lysates from 293 T cells transfected with empty vector (EV) or Flag-RNF152 were subjected to immunoprecipitation (IP) with Flag M2 beads, followed by immunoblotting with indicated antibodies. c The cell lysates of MG132-treated HuH6 cells were subjected to immunoprecipitation with IgG or anti-RNF152 antibody and detected by immunoblotting with indicated antibodies. d 293 T cells transfected Flag-tagged TSPAN12 plasmid were lysed and incubated with GST alone or GST-RNF152 immobilized on GST-Sepharose beads. The bound proteins were then detected by immunoblotting with indicated antibodies