R1609P

antibody from Acris Antibodies GmbH

Targeting: ING3 Eaf4, FLJ20089, MEAF4, p47ING3

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Antibody Data

Product number

R1609P

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#R1609P, RRID:AB_1004184

Product name

anti ING3

Antibody type

Polyclonal

Antigen

Synthetic peptide corresponding aa 294-304 of Human ING3 protein (Inhibitor of growth family, member 3). This sequence only shows homology to isoform 1 for ING3

Reactivity

Human, Mouse, Rat, Canine, Chicken/Avian, Simian

Host

Goat

Vial size

0.1 mg

Concentration

1.1 mg/ml (UVabs. at 280 nm)

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot using R1609P ING3 antibody shows detection of a band at ~55 kDa corresponding to ING3 in RKO cells transfected with ING3 (lane 2). Control RKO cells do not show detection of this specific band (lane 1). The identity of the non-specific bands at 33 kDa and 20 kDa has not been determined. Each lane contains approximately 10 µg of RKO whole cell lysate (ATCC#CRL-2577-Human colon cancer) separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred to nitrocellulose. After blocking with 5% NF dry milk, the membrane was probed with the primaryantibody diluted to 1:1,000. Incubation was at 4° C overnight followed by washes and reaction with a 1:20,000 dilution of IRDye™800 conjugated Rb-a-Goat IgG [H&L] MXHu for 45 min at room temperature. IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yieldsimilar results.