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- Antibody Data
- Antigen structure
- References [13]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- 17006-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#17006-1-AP, RRID:AB_2301068
- Product name
- MRPS15 antibody
- Antibody type
- Polyclonal
- Description
- MRPS15 antibody (Cat. #17006-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Identification of TMEM126A as OXA1L-interacting protein reveals cotranslational quality control in mitochondria.
Four-Color STED Super-Resolution RNA Fluorescent In Situ Hybridization and Immunocytochemistry to Visualize Mitochondrial mRNAs in Context with Mitochondrial Ribosomes.
Non-canonical phosphoglycerate dehydrogenase activity promotes liver cancer growth via mitochondrial translation and respiratory metabolism.
Linear Density Sucrose Gradients to Study Mitoribosomal Biogenesis in Tissue-Specific Knockout Mice.
An in vitro system to silence mitochondrial gene expression.
Differential processing and localization of human Nocturnin controls metabolism of mRNA and nicotinamide adenine dinucleotide cofactors.
MitoRibo-Tag Mice Provide a Tool for In Vivo Studies of Mitoribosome Composition.
Mutations in the MRPS28 gene encoding the small mitoribosomal subunit protein bS1m in a patient with intrauterine growth retardation, craniofacial dysmorphism and multisystemic involvement.
The human Obg protein GTPBP10 is involved in mitoribosomal biogenesis.
Stomatin-like protein 2 deficiency results in impaired mitochondrial translation.
Mitochondrial Protein Synthesis Adapts to Influx of Nuclear-Encoded Protein.
Initial steps in RNA processing and ribosome assembly occur at mitochondrial DNA nucleoids.
Mitochondrial ribosomal RNA (rRNA) methyltransferase family members are positioned to modify nascent rRNA in foci near the mitochondrial DNA nucleoid.
Poerschke S, Oeljeklaus S, Cruz-Zaragoza LD, Schenzielorz A, Dahal D, Hillen HS, Das H, Kremer LS, Valpadashi A, Breuer M, Sattmann J, Richter-Dennerlein R, Warscheid B, Dennerlein S, Rehling P
Molecular cell 2024 Jan 18;84(2):345-358.e5
Molecular cell 2024 Jan 18;84(2):345-358.e5
Four-Color STED Super-Resolution RNA Fluorescent In Situ Hybridization and Immunocytochemistry to Visualize Mitochondrial mRNAs in Context with Mitochondrial Ribosomes.
Albus CA, Berlinguer-Palmini R, Chrzanowska-Lightowlers ZM, Lightowlers RN
Methods in molecular biology (Clifton, N.J.) 2023;2661:303-316
Methods in molecular biology (Clifton, N.J.) 2023;2661:303-316
Non-canonical phosphoglycerate dehydrogenase activity promotes liver cancer growth via mitochondrial translation and respiratory metabolism.
Shu Y, Hao Y, Feng J, Liu H, Li ST, Feng J, Jiang Z, Ye L, Zhou Y, Sun Y, Zhou Z, Wei H, Gao P, Zhang H, Sun L
The EMBO journal 2022 Dec 1;41(23):e111550
The EMBO journal 2022 Dec 1;41(23):e111550
Linear Density Sucrose Gradients to Study Mitoribosomal Biogenesis in Tissue-Specific Knockout Mice.
Ruzzenente B, Metodiev MD
Methods in molecular biology (Clifton, N.J.) 2021;2224:47-60
Methods in molecular biology (Clifton, N.J.) 2021;2224:47-60
An in vitro system to silence mitochondrial gene expression.
Cruz-Zaragoza LD, Dennerlein S, Linden A, Yousefi R, Lavdovskaia E, Aich A, Falk RR, Gomkale R, Schöndorf T, Bohnsack MT, Richter-Dennerlein R, Urlaub H, Rehling P
Cell 2021 Nov 11;184(23):5824-5837.e15
Cell 2021 Nov 11;184(23):5824-5837.e15
Differential processing and localization of human Nocturnin controls metabolism of mRNA and nicotinamide adenine dinucleotide cofactors.
Abshire ET, Hughes KL, Diao R, Pearce S, Gopalakrishna S, Trievel RC, Rorbach J, Freddolino PL, Goldstrohm AC
The Journal of biological chemistry 2020 Oct 30;295(44):15112-15133
The Journal of biological chemistry 2020 Oct 30;295(44):15112-15133
MitoRibo-Tag Mice Provide a Tool for In Vivo Studies of Mitoribosome Composition.
Busch JD, Cipullo M, Atanassov I, Bratic A, Silva Ramos E, Schöndorf T, Li X, Pearce SF, Milenkovic D, Rorbach J, Larsson NG
Cell reports 2019 Nov 5;29(6):1728-1738.e9
Cell reports 2019 Nov 5;29(6):1728-1738.e9
Mutations in the MRPS28 gene encoding the small mitoribosomal subunit protein bS1m in a patient with intrauterine growth retardation, craniofacial dysmorphism and multisystemic involvement.
Pulman J, Ruzzenente B, Bianchi L, Rio M, Boddaert N, Munnich A, Rötig A, Metodiev MD
Human molecular genetics 2019 May 1;28(9):1445-1462
Human molecular genetics 2019 May 1;28(9):1445-1462
The human Obg protein GTPBP10 is involved in mitoribosomal biogenesis.
Lavdovskaia E, Kolander E, Steube E, Mai MM, Urlaub H, Richter-Dennerlein R
Nucleic acids research 2018 Sep 19;46(16):8471-8482
Nucleic acids research 2018 Sep 19;46(16):8471-8482
Stomatin-like protein 2 deficiency results in impaired mitochondrial translation.
Mitsopoulos P, Lapohos O, Weraarpachai W, Antonicka H, Chang YH, Madrenas J
PloS one 2017;12(6):e0179967
PloS one 2017;12(6):e0179967
Mitochondrial Protein Synthesis Adapts to Influx of Nuclear-Encoded Protein.
Richter-Dennerlein R, Oeljeklaus S, Lorenzi I, Ronsör C, Bareth B, Schendzielorz AB, Wang C, Warscheid B, Rehling P, Dennerlein S
Cell 2016 Oct 6;167(2):471-483.e10
Cell 2016 Oct 6;167(2):471-483.e10
Initial steps in RNA processing and ribosome assembly occur at mitochondrial DNA nucleoids.
Bogenhagen DF, Martin DW, Koller A
Cell metabolism 2014 Apr 1;19(4):618-29
Cell metabolism 2014 Apr 1;19(4):618-29
Mitochondrial ribosomal RNA (rRNA) methyltransferase family members are positioned to modify nascent rRNA in foci near the mitochondrial DNA nucleoid.
Lee KW, Okot-Kotber C, LaComb JF, Bogenhagen DF
The Journal of biological chemistry 2013 Oct 25;288(43):31386-99
The Journal of biological chemistry 2013 Oct 25;288(43):31386-99
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- human brain tissue were subjected to SDS PAGE followed by western blot with 17006-1-AP(MRPS15 antibody) at dilution of 1:500
- Sample type
- tissue
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using MRPS15 antibody 17006-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human spleen using 17006-1-AP(MRPS15 antibody) at dilution of 1:100 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human spleen using 17006-1-AP(MRPS15 antibody) at dilution of 1:100 (under 40x lens)
- Sample type
- tissue
