Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-31148 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SMNDC1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: NT2D1, IMR32, U87-MG, MCF-7. Predicted reactivity: Mouse (99%), Rat (99%), Pig (98%), Rhesus Monkey (99%), Bovine (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SMNDC1 using 30 µg of IMR32 lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a SMNDC1 polyclonal antibody (Product # PA5-31148) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of SMNDC1 was performed by separating 30 µg of various whole cell extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a SMNDC1 Polyclonal Antibody (Product # PA5-31148) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of SMNDC1 in paraformaldehyde-fixed MCF-7 cells using a SMNDC1 polyclonal antibody (Product # PA5-31148) at a 1:500 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using SMNDC1 (Product # PA5-31148) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SMNDC1 Polyclonal Antibody detects SMNDC1 protein at nucleus in human lung cancer by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. SMNDC1 Polyclonal Antibody (Product # PA5-31148) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded SNU1 xenograft, using SMNDC1 (Product # PA5-31148) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.