AF3084

antibody from R&D Systems

Targeting: FAM3C GS3876, ILEI

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF3084

Provider

R&D Systems

Product name

Human FAM3C Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects human FAM3C in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 50% cross-reactivity with recombinant mouse FAM3C is observed and 5% cross-reactivity with recombinant human (rh) FAM3A, rhFAM3B, and rhFAM3D is observed.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Antigen sequence

Q92520


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human FAM3C by Western Blot. Western blot shows lysates of JEG-3 human epithelial choriocarcinoma cell line and HCT-116 human colorectal carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human FAM3C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3084) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for FAM3C at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.