Antibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Immunocytochemistry [1]
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Validation data
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- Product number
- MAB3084 - Provider product page
- Provider
- R&D Systems
- Product name
- Human FAM3C Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human FAM3C in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) FAM3A, rhFAM3B, recombinant mouse (rm) FAM3C, or rmFAM3D is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q92520
- Isotype
- IgG
- Antibody clone number
- 438618
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- FAM3C in A549 Human Cell Line. FAM3C was detected in immersion fixed A549 human lung carcinoma cells treated with 10ng/mL of Recombinant Human TGF-beta 1 (Catalog # 240-B) for 48 hours using Mouse Anti-Human FAM3C Monoclonal Antibody (Catalog # MAB3084) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counter-stained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.