Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AGC-020-25UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NMDAR2D (GRIN2D) (extracellular) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Reconstitution: 1 X 25 µL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 25 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain membranes: - 1,2. Anti-NMDAR2D (GRIN2D) (extracellular) Antibody (#AGC-020), (1:200).3,4. Anti-NMDAR2D (GRIN2D) (extracellular) Antibody , preincubated with NMDAR2D/GRIN2D (extracellular) Blocking Peptide (#BLP-GC020).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of NMDA receptor 2D in rat reticular thalamic nucleus - Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-NMDAR2D (GRIN2D) (extracellular) Antibody (#AGC-020), (1:200). A. Staining (green) appears in a discreet population of cells (thin arrows). B. The same section was stained for parvalbumin (red) and cell nuclei were visualized with DAPI (blue). Cells positive for NR2D but negative for parvalbumin (thick arrow, shown in A) and cells positive for both NR2D and parvalbumin (vertical arrow) are both present.