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LearnPA5-30863
antibody from Invitrogen Antibodies
Targeting: HIP1R
FLJ14000, HIP12, HIP3, ILWEQ, KIAA0655
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
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- Product number
- PA5-30863 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HIP1R Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: MCF-7. Predicted reactivity: Mouse (96%), Rat (97%), Xenopus laevis (89%), Rabbit (95%), Chicken (90%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using HIP1R Polyclonal Antibody (Product # PA5-30863). Sample (30 µg of whole cell lysate). Lane A: MCF-7 . 7.5% SDS PAGE. HIP1R Polyclonal Antibody (Product # PA5-30863) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HIP1 Related antibody [N1N2], N-term detects HIP1R protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HIP1R protein stained by HIP1 Related antibody [N1N2], N-term (Product # PA5-30863) diluted at 1:500. Blue: Hoechst 33343 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HIP1 Related antibody [N1N2], N-term detects HIP1R protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HIP1R protein stained by HIP1 Related antibody [N1N2], N-term (Product # PA5-30863) diluted at 1:500. Blue: Hoechst 33343 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of HIP1R was performed in paraffin-embedded rat brain tissue using HIP1R Polyclonal Antibody (Product # PA5-30863) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using HIP1 Related (Product # PA5-30863) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
