PA5-57636

antibody from Invitrogen Antibodies

Targeting: DNAH6 Dnahc6, DNHL1, FLJ37357, HL-2

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-57636

Provider

Invitrogen Antibodies

Product name

DNAH6 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: TKLNTILCQT FVFCYLWSLG GNLTENYYDS FDTFIRTQFD DNPDARLPNS GDLWSIHMDF DTKRLDPWER IIPTFKYNRD VP Highest antigen sequence identity to the following orthologs: Mouse - 93%, Rat - 89%.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.2 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

G-quadruplexes originating from evolutionary conserved L1 elements interfere with neuronal gene expression in Alzheimer's disease.

Hanna R, Flamier A, Barabino A, Bernier G
Nature communications 2021 Mar 23;12(1):1828

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of DNAH6 in human cell line A-431 shows positivity in plasma membrane, cytoplasm & nucleus but excluded from the nucleoli. Samples were probed using a DNAH6 Polyclonal Antibody (Product # PA5-57636).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 5 1H6 peaks are enriched at key regulatory elements of neuronal genes. a Venn diagram and Gene Ontology analysis of genes associated with peaks common to both Ctrl2 and AD1 neurons or specific to AD neurons (AD1). The most significant pathways are indicated on the diagram along with the respective P value calculated by the GO algorithm. b Sashimi plot of three different loci containing a 1H6 peak and associated with a differential splicing event. Exons are represented as blue boxes. The red lines represent a peak found in AD neurons; the green lines represent a peak found in control (Ctrl) neurons green. Loss of the intragenic peak in AD neurons between the SMN1 and NAIP genes is associated with the formation of cryptic fusion transcripts. The common peak found at the APP locus (but increased in AD) is associated with loss of some minor isoforms in AD neurons. The unique peak gained in AD neurons at the DNAH6 locus is associated with loss of minor isoforms and a large splicing gap in AD neurons. c Table showing the number of genes containing a 1H6 peak, as well as the number of genes that entailed a differential splicing event associated with a 1H6 peak when comparing Ctrl1 and Ctrl2 neurons with AD1 neurons. The splicing events were subdivided into loss of a minor isoform or formation of a gap junction. d Western blot analyses of Ctrl2 and AD2 neurons. GAPDH and ponceau were used as normalizers. Black arrows indicate differentially expressed protein variants. e Volcano