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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- PA5-34823 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- UNG Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLa, HeLa transfect shUNG #1, HeLa transfect shUNG #2.
- Concentration
- 0.71 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of UNG using A) 30 µg HeLa whole cell lysate (B) 30 µg whole cell lysate of UNG shRNA#1-transfected HeLa cells and C) 30 µg whole cell lysate of UNG shRNA2-transfected HeLa cells. Samples were loaded onto a 11% SDS-PAGE gel and probed with a UNG polyclonal antibody (Product # PA5-34823) at a dilution of 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- UNG Polyclonal Antibody detects UNG protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min. Green: UNG protein stained by UNG Polyclonal Antibody (Product # PA5-34823) diluted at 1:500. Blue: Hoechst 33342 staining.
