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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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Validation data
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- Product number
- PA5-30643 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DNALI1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A549. Predicted reactivity: Mouse (98%), Rat (99%), Zebrafish (83%), Xenopus laevis (90%), Bovine (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.9 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Post-translational polymodification of β1-tubulin regulates motor protein localisation in platelet production and function.
Khan AO, Slater A, Maclachlan A, Nicolson PLR, Pike JA, Reyat JS, Yule J, Stapley R, Rayes J, Thomas SG, Morgan NV
Haematologica 2022 Jan 1;107(1):243-259
Haematologica 2022 Jan 1;107(1):243-259
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using DNALI1 Polyclonal Antibody (Product # PA5-30643). Sample (30 µg of whole cell lysate). Lane A: A549. 12% SDS PAGE. DNALI1 Polyclonal Antibody (Product # PA5-30643) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of DNALI1 in methanol-fixed A549 cells using a DNALI1 polyclonal antibody (Product # PA5-30643) at a 1:500 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of DNALI1 was performed in paraffin-embedded mouse muscle tissue using DNALI1 Polyclonal Antibody (Product # PA5-30643) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of DNALI1 was performed in paraffin-embedded mouse testis tissue using DNALI1 Polyclonal Antibody (Product # PA5-30643) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- DNALI1 Polyclonal Antibody detects DNALI1 protein at cytosol on BT483 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded BT483 xenograft. DNALI1 Polyclonal Antibody (Product # PA5-30643) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
