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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunohistochemistry [2]
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- Product number
- GTX129726 - Provider product page
- Provider
- GeneTex
- Product name
- DNA polymerase beta antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- DNA polymerase beta antibody detects DNA polymerase beta protein by western blot analysis.A. 30 £gg 293T whole cell lysate/extract B. 30 £gg whole cell lysate/extract of human POLB-transfected 293T cells10 % SDS-PAGEDNA polymerase beta antibody (GTX129726) dilution: 1:5000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and DNA polymerase beta knockout (KO) HeLa cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with DNA polymerase beta antibody (GTX129726) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and DNA polymerase beta knockout (KO) HeLa cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with DNA polymerase beta antibody (GTX129726) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) unboiled 293T whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with DNA polymerase beta antibody (GTX129726) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- DNA polymerase beta antibody detects DNA polymerase beta protein at cytoplasm and nucleus on human lung carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung carcinoma. DNA polymerase beta antibody (GTX129726) diluted at 1:250.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- DNA polymerase beta antibody detects DNA polymerase beta protein at nucleus on mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. DNA polymerase beta antibody (GTX129726) dilution: 1:500.
