- MA5-12086 - Invitrogen Antibodies POLB antibody

Submitted references Next generation high throughput DNA damage detection platform for genotoxic compound screening.

Sykora P, Witt KL, Revanna P, Smith-Roe SL, Dismukes J, Lloyd DG, Engelward BP, Sobol RW
Scientific reports 2018 Feb 9;8(1):2771

HSP90 regulates DNA repair via the interaction between XRCC1 and DNA polymerase β.

Fang Q, Inanc B, Schamus S, Wang XH, Wei L, Brown AR, Svilar D, Sugrue KF, Goellner EM, Zeng X, Yates NA, Lan L, Vens C, Sobol RW
Nature communications 2014 Nov 26;5:5513

Transcriptional profiling reveals elevated Sox2 in DNA polymerase ß null mouse embryonic fibroblasts.

Li J, Luthra S, Wang XH, Chandran UR, Sobol RW
American journal of cancer research 2012;2(6):699-713

N-methylpurine DNA glycosylase and DNA polymerase beta modulate BER inhibitor potentiation of glioma cells to temozolomide.

Tang JB, Svilar D, Trivedi RN, Wang XH, Goellner EM, Moore B, Hamilton RL, Banze LA, Brown AR, Sobol RW
Neuro-oncology 2011 May;13(5):471-86

The base excision repair pathway is required for efficient lentivirus integration.

Yoder KE, Espeseth A, Wang XH, Fang Q, Russo MT, Lloyd RS, Hazuda D, Sobol RW, Fishel R
PloS one 2011 Mar 23;6(3):e17862

Parp1 activation in mouse embryonic fibroblasts promotes Pol beta-dependent cellular hypersensitivity to alkylation damage.

Jelezcova E, Trivedi RN, Wang XH, Tang JB, Brown AR, Goellner EM, Schamus S, Fornsaglio JL, Sobol RW
Mutation research 2010 Apr 1;686(1-2):57-67

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on whole cell extracts (30 µg lysate) of Daudi (Lane 1), K-562 (Lane 2), NIH/3T3 (Lane3), SH-SY5Y (Lane4) and tissue extract (30 µg lysate) of Rat Testis (Lane 5).The blots were probed with Anti-DNA Polymerase beta Mouse monoclonal Antibody (Product # MA5-12086, 2 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjµgate (Product # A28177, 0.4 µg/mL, 1:2500 dilution). A 38 kDa band corresponding to DNA Polymerase beta was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10 % Bis-Tris gel (Product # NP0302BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot of DNA Polymerase beta using DNA Polymerase beta Monoclonal Antibody (Product # MA5-12086) on HeLa Cells.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of POLB was achieved by transfecting SH-SY5Y cells with POLB specific siRNAs (Silencer® select Product # s10775). Western blot analysis (Fig. a) was performed using whole cell extracts from the POLB knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with POLB Monoclonal Antibody (61) (Product # MA5-12086, 1:1000 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25µg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Loss of signal upon siRNA mediated knock down confirms that antibody is specific to POLB.

MA5-12086

Antibody data