PA5-22143

antibody from Invitrogen Antibodies

Targeting: NDUFA5 B13, CI-13kB, CI-13KD-B, NUFM, UQOR13

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-22143

Provider

Invitrogen Antibodies

Product name

NDUFA5 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Recommended positive controls: H1299, HeLa, HepG2, mouse brain. Predicted reactivity: Mouse (82%), Rat (80%), Rhesus Monkey (94%), Chimpanzee (100%), Bovine (87%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Comparative Proteomic Profiling Identifies Reciprocal Expression of Mitochondrial Proteins Between White and Gray Matter Lesions From Multiple Sclerosis Brains.

Rai NK, Singh V, Li L, Willard B, Tripathi A, Dutta R
Frontiers in neurology 2021;12:779003

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using NDUFA5 Polyclonal Antibody (Product # PA5-22143). Sample (30 µg of whole cell lysate). Lane A: Hep G2 . 15% SDS PAGE. NDUFA5 Polyclonal Antibody (Product # PA5-22143) diluted at 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using NDUFA5 Polyclonal Antibody (Product # PA5-22143). Sample (50 µg of whole cell lysate). Lane A: mouse brain . 15% SDS PAGE. NDUFA5 Polyclonal Antibody (Product # PA5-22143) diluted at 1:1,000.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of NDUFA5 was performed in paraffin-embedded human colon carcinoma tissue using NDUFA5 Polyclonal Antibody (Product # PA5-22143) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using NDUFA5 (Product # PA5-22143) antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using NDUFA5 (Product # PA5-22143) antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 3 Gene enrichment analysis and validation of WML DEPs. (A) Bar graphs showing cellular component (red) and biological process (blue) enrichment of upregulated WML proteomes. (B) Venn diagram showing a comparison between mitochondrial genes from the mitocarta 2.0 dataset and upregulated proteins identified in our study. (C) LFQ-based heatmap showing upregulated mitochondrial protein distribution across different mitochondrial complexes. (D) Bar graphs showing cellular component (red) and biological process (blue) enrichment of downregulated WML proteomes. (E) LFQ-based heat map showing downregulated proteins related to microfilament proteins, microtubule proteins, and cytoplasmic dynein dynactin complex proteins. (F-L) . Representative IHC images showing cellular localization of VDAC1, NDUFA5, and ATP5L (G-L) WMLs and NAWM from progressive MS brains. Scale bar- 500mum (F) and 20mum (G-L) .

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 5 Cortical GML proteome profiling. (A) Volcano plot displaying the distribution of all proteins ( n = 1182) with relative protein abundance (Log2 fold change) plotted against significance level (-Log 10 P -Value). Significantly upregulated (red circle) as well as downregulated (blue circle) proteins in GMLs are also indicated. (B) Cellular identity of the significantly dysregulated GML proteins (upregulated and downregulated). (C) Venn diagram comparison of DEPs ( p < 0.05) between WMLs and GMLs. (D) IPA canonical pathways, activated (red) and inhibited (blue), associated with significantly dysregulated proteins in GMLs. (E) LFQ-based heat map of mitochondria-specific downregulated proteins in GMLs. Proteins that are common and have opposite expressions in WML proteomic data are highlighted in red. (F) PPI network analysis of downregulated proteins showing enrichment of mitochondria-related pathways. (G) MCODE analysis revealed a highly interconnected sub-network associated with oxidative phosphorylation. (H-L) PLP- stained image of (H) sub-pial lesion. IHC images showing reduced expression of NDUFA5 (I,J) and ATP5L (K,L) in demyelinated regions (red box) compared to NAGM (blue box). Scale bar- 500mum (H) and 100mum (I-L) .