PA5-116790

antibody from Invitrogen Antibodies

Targeting: RALGDS RalGEF, RGDS, RGF

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-116790

Provider

Invitrogen Antibodies

Product name

RALGDS Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Positive Samples: U-87MG, Mouse ovary, Mouse lung Immunogen sequence: CNSSSALPLY NQQVGDCCII RVSLDVDNGN MYKSILVTSQ DKAPAVIRKA MDKHNLEEEE PEDYELLQIL SDDRKLKIPE NANVFYAMNS TANYDFVLKK RTFTKGVKVK HGASSTLPRM KQKGLKIAKG IF

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.04 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of RALGDS in extracts of various cell lines. Samples were incubated with RALGDS Polyclonal antibody (Product # PA5-116790) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of RALGDS in L929 cells. Samples were incubated with RALGDS Polyclonal antibody (Product # PA5-116790) using a dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence analysis of RALGDS in U-2 OS cells using RALGDS Polyclonal Antibody (Product # PA5-116790) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of RALGDS in U-2 OS cells. Samples were incubated with RALGDS Polyclonal antibody (Product # PA5-116790) using a dilution of 1:100. Blue: DAPI for nuclear staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of RALGDS in paraffin-embedded human thyroid cancer. Samples were incubated with RALGDS Polyclonal antibody (Product # PA5-116790) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of RALGDS in paraffin-embedded mouse testis. Samples were incubated with RALGDS Polyclonal antibody (Product # PA5-116790) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of RALGDS in paraffin-embedded rat brain. Samples were incubated with RALGDS Polyclonal antibody (Product # PA5-116790) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of RALGDS in paraffin-embedded rat brain. Samples were incubated with RALGDS Polyclonal antibody (Product # PA5-116790) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.