Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-49724 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MMP27 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- The antibody detects endogenous level of total MMP27 protein.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Mmp27 was performed by loading Mouse brain tissue lysate (80µg) on to a 10% SDS-PAGE gel. Proteins were transferred to a membrane and the membrane was probed with a Mmp27 antibody (Product # PA5-49724) at a 1/500 dilution for 2 minutes, followed by Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of MMP27 in paraffin-embedded human breast cancer tissue using (left) MMP27 Polyclonal Antibody (Product # PA5-49724) at a 1:40 dilution and (right) is treated with the synthetic peptide.