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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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- Product number
- MAB10129-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human alpha-2A Adrenergic R/ADRA2A Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human alpha-2A Adrenergic R/ADRA2A in direct ELISAs.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- 1006614
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of ADRA2A in HEK293 Human Cell Line Transfected with Human ADRA2A and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human ADRA2A or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human ADRA2A Monoclonal Antibody (Catalog # MAB10129) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.
