PA5-120835

antibody from Invitrogen Antibodies

Targeting: ADRA2A ADRA2, ADRA2R, ADRAR

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-120835

Provider

Invitrogen Antibodies

Product name

alpha-2a Adrenergic Receptor Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Positive test controls include: K-562, Mouse brain, Mouse Spinal cord. The target is usually found in the following locations: Cell membrane, Multi-pass membrane protein. Immunogen sequence: RRTRVPPSRR GPDAVAAPPG GTERRPNGLG PERSAGPGGA EAEPLPTQLN GAPGEPAPAG PRDTDALDLE ESSSSDHAER PPGPRRPERG PRGKGKARAS QVKPGDSLPR RGPGATGIGT PAAGPGEERV GAAKASRWRG R

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.59 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of alpha-2a Adrenergic Receptor in extracts of various cell lines. Samples were incubated with alpha-2a Adrenergic Receptor Polyclonal antibody (Product # PA5-120835) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of alpha-2a Adrenergic Receptor in C6 cells using a alpha-2a Adrenergic Receptor Polyclonal antibody (Product # PA5-120835). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of alpha-2a Adrenergic Receptor in C6 cells using a alpha-2a Adrenergic Receptor Polyclonal antibody (Product # PA5-120835). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of alpha-2a Adrenergic Receptor in C6 cells using a alpha-2a Adrenergic Receptor Polyclonal antibody (Product # PA5-120835). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of alpha-2a Adrenergic Receptor in C6 cells using a alpha-2a Adrenergic Receptor Polyclonal antibody (Product # PA5-120835). Blue: DAPI for nuclear staining.