Antibody data
- Antibody Data
 - Antigen structure
 - References [3]
 - Comments [0]
 - Validations
 - Western blot [1]
 - Immunohistochemistry [1]
 
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Validation data
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- Product number
 - NB100-57094 - Provider product page

 - Provider
 - Novus Biologicals
 - Proper citation
 - Novus Cat#NB100-57094, RRID:AB_2199372
 - Product name
 - Goat Polyclonal TBC1D4 Antibody
 - Antibody type
 - Polyclonal
 - Description
 - Immunogen affinity purified.
 - Reactivity
 - Human
 - Host
 - Goat
 - Antigen sequence
 DDPEKIEERKKSK- Isotype
 - IgG
 - Vial size
 - 0.1 mg
 - Concentration
 - 0.5 mg/ml
 - Storage
 - Store at -20C. Avoid freeze-thaw cycles.
 
Submitted references		Regulation of glucose transporter 4 translocation by the Rab guanosine triphosphatase-activating protein AS160/TBC1D4: role of phosphorylation and membrane association.
				
Insulin-stimulated phosphorylation of the Akt substrate AS160 is impaired in skeletal muscle of type 2 diabetic subjects.
				
Insulin-stimulated phosphorylation of the Akt substrate AS160 is impaired in skeletal muscle of type 2 diabetic subjects.
				
		
	
			Stöckli J, Davey JR, Hohnen-Behrens C, Xu A, James DE, Ramm G
Molecular endocrinology (Baltimore, Md.) 2008 Dec;22(12):2703-15
		Molecular endocrinology (Baltimore, Md.) 2008 Dec;22(12):2703-15
Insulin-stimulated phosphorylation of the Akt substrate AS160 is impaired in skeletal muscle of type 2 diabetic subjects.
			Karlsson HK, Zierath JR, Kane S, Krook A, Lienhard GE, Wallberg-Henriksson H
Diabetes 2005 Jun;54(6):1692-7
		Diabetes 2005 Jun;54(6):1692-7
Insulin-stimulated phosphorylation of the Akt substrate AS160 is impaired in skeletal muscle of type 2 diabetic subjects.
			Karlsson HK, Zierath JR, Kane S, Krook A, Lienhard GE, Wallberg-Henriksson H
Diabetes 2005 Jun;54(6):1692-7
		Diabetes 2005 Jun;54(6):1692-7
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					Supportive validation
					
									
				
				- Submitted by
 - Novus Biologicals (provider)
 - Main image
 
- Experimental details
 - Western Blot: TBC1D4 Antibody [NB100-57094] - (0.1ug/ml) staining of Daudi cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
 
							
					Supportive validation
					
									
				
		- Submitted by
 - Novus Biologicals (provider)
 - Main image
 
- Experimental details
 - Immunohistochemistry-Paraffin: TBC1D4 Antibody [NB100-57094] - (3.8ug/ml) staining of Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.