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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [2]
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Validation data
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- Product number
- TA502695 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA502695, RRID:AB_11125672
- Product name
- TBC1D4 mouse monoclonal antibody, clone OTI5E6 (formerly 5E6)
- Antibody type
- Monoclonal
- Description
- TBC1D4 mouse monoclonal antibody, clone OTI5E6 (formerly 5E6)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- TBC1D4
- Isotype
- IgG
- Antibody clone number
- OTI5E6
- Vial size
- 100 µl
- Concentration
- 0.35 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TBC1D4 (RC212105, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TBC1D4.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-TBC1D4 mouse monoclonal antibody (TA502695) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TBC1D4(RC212105).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC212105 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-TBC1D4 antibody(TA502695), and then analyzed by flow cytometry.
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Jurkat cells, using anti-TBC1D4 antibody(TA502695),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
- Validation comment
- FC
