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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
- Other assay [1]
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- Product number
- MA5-14840 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- AS160 Monoclonal Antibody (G.853.7)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- It is not recommended to aliquot this antibody.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- G.853.7
- Vial size
- 100 µL
- Concentration
- 81 µg/mL
- Storage
- -20°C
Submitted references The Dysregulation of OGT/OGA Cycle Mediates Tau and APP Neuropathology in Down Syndrome.
Adipose Tissue Insulin Resistance in Gestational Diabetes.
Zuliani I, Lanzillotta C, Tramutola A, Francioso A, Pagnotta S, Barone E, Perluigi M, Di Domenico F
Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics 2021 Jan;18(1):340-363
Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics 2021 Jan;18(1):340-363
Adipose Tissue Insulin Resistance in Gestational Diabetes.
Tumurbaatar B, Poole AT, Olson G, Makhlouf M, Sallam HS, Thukuntla S, Kankanala S, Ekhaese O, Gomez G, Chandalia M, Abate N
Metabolic syndrome and related disorders 2017 Mar;15(2):86-92
Metabolic syndrome and related disorders 2017 Mar;15(2):86-92
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of AS160 in extracts from HepG2 and RD cells using AS160 monoclonal antibody (Product # MA5-14840).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-AS160 Monoclonal Antibody (G.853.7) (Product # MA5-14840) and a 146 kDa band corresponding to AS160 was observed in cell lines tested. Whole cell extracts (40 µg lysate) of Caco-2 (Lane 1) and HEK-293 (Lane 2) were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
