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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
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- Product number
- HPA030383 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA030383, RRID:AB_10599977
- Product name
- Anti-KCMF1
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
LSGVRRSAGGQLNSSGPSASQLQQLQMQLQLERQH
AQAARQQLETARNATRRTNTSSVTTTITQSTATTN
IANTESSQQTLQNSQFLLTRL- Isotype
- IgG
- Vial size
- 100 µl
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Submitted references Stress response silencing by an E3 ligase mutated in neurodegeneration
N-terminal acetylation shields proteins from degradation and promotes age-dependent motility and longevity
Haakonsen D, Heider M, Ingersoll A, Vodehnal K, Witus S, Uenaka T, Wernig M, Rapé M
Nature 2024;626(8000):874-880
Nature 2024;626(8000):874-880
N-terminal acetylation shields proteins from degradation and promotes age-dependent motility and longevity
Varland S, Silva R, Kjosås I, Faustino A, Bogaert A, Billmann M, Boukhatmi H, Kellen B, Costanzo M, Drazic A, Osberg C, Chan K, Zhang X, Tong A, Andreazza S, Lee J, Nedyalkova L, Ušaj M, Whitworth A, Andrews B, Moffat J, Myers C, Gevaert K, Boone C, Martinho R, Arnesen T
Nature Communications 2023;14(1)
Nature Communications 2023;14(1)
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Enhanced validation
- Submitted by
- klas2
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot of cell lysate from U-2 OS cells transfected with either siRNA targeting KCMF1 or control siRNA. Lane 1: Marker (250, 130, 95, 72, 55, 36, 28, 17, 10) Lane 2: Cell lysate from U-2OS cells transfected with siRNA targeting KCMF1 Lane 3: N/A Lane 4: Cell lysate from U-2OS cells transfected with control siRNA Right image, lane 1-4: loading control
- Sample type
- U-2 OS
- Primary Ab dilution
- 1:469
- Conjugate
- Horseradish Peroxidase
- Secondary Ab
- Secondary Ab
- Secondary Ab dilution
- 1:3000
- Knockdown/Genetic Approaches Application
- Western blot
