Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [11]
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Validation data
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- Product number
- NBP1-84284 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-84284, RRID:AB_11015237
- Product name
- Rabbit Polyclonal KCMF1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human KCMF1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Western Blot: KCMF1 Antibody [NBP1-84284] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: KCMF1 Antibody [NBP1-84284] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: KCMF1 Antibody [NBP1-84284] - Staining of human colon shows moderate cytoplasmic positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: KCMF1 Antibody [NBP1-84284] - Staining of human colon, liver, pancreas and testis using Anti-KCMF1 antibody NBP1-84284 (A) shows similar protein distribution across tissues to independent antibody NBP1-84283 (B).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: KCMF1 Antibody [NBP1-84284] - Staining of human pancreas.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: KCMF1 Antibody [NBP1-84284] - Staining of human liver.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: KCMF1 Antibody [NBP1-84284] - Staining of human testis.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: KCMF1 Antibody [NBP1-84284] - Staining of human colon.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: KCMF1 Antibody [NBP1-84284] - Staining of human kidney shows weak to moderate cytoplasmic positivity in cells in tubules.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: KCMF1 Antibody [NBP1-84284] - Staining of human liver shows no cytoplasmic postivity in hepatocytes as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: KCMF1 Antibody [NBP1-84284] - Staining of human testis shows weak to moderate cytoplasmic postivity in cells in seminiferous ducts.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: KCMF1 Antibody [NBP1-84284] - Staining of human colon shows moderate cytoplasmic positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: KCMF1 Antibody [NBP1-84284] - Staining of human colon, kidney, liver and testis using Anti-KCMF1 antibody NBP1-84284 (A) shows similar protein distribution across tissues to independent antibody NBP1-84283 (B).