PA5-56366

antibody from Invitrogen Antibodies

Targeting: YRDC FLJ23476, IRIP, SUA5

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-56366

Provider

Invitrogen Antibodies

Product name

YRDC Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: ASSLNVEEFQ DLWPQLSLVI DGGQIGDGQS PECRLGSTVV DLSVPGKFGI IRPGCALEST TAILQQKYGL LP Highest antigen sequence identity to the following orthologs: Mouse - 92%, Rat - 89%.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.2 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Defects in t(6)A tRNA modification due to GON7 and YRDC mutations lead to Galloway-Mowat syndrome.

Arrondel C, Missoury S, Snoek R, Patat J, Menara G, Collinet B, Liger D, Durand D, Gribouval O, Boyer O, Buscara L, Martin G, Machuca E, Nevo F, Lescop E, Braun DA, Boschat AC, Sanquer S, Guerrera IC, Revy P, Parisot M, Masson C, Boddaert N, Charbit M, Decramer S, Novo R, Macher MA, Ranchin B, Bacchetta J, Laurent A, Collardeau-Frachon S, van Eerde AM, Hildebrandt F, Magen D, Antignac C, van Tilbeurgh H, Mollet G
Nature communications 2019 Sep 3;10(1):3967

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of YRDC in human cell line U-2 OS shows positivity in mitochondria. Samples were probed using a YRDC Polyclonal Antibody (Product # PA5-56366).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 3 Effects of YRDC and GON7 mutations on t 6 A biosynthesis. a Evaluation of fitness of Deltasua5 yeast strains expressing human YRDC variants (spots are from 10-fold serial dilutions of cell suspensions at OD 600nm = 0.5, and three independent clones were evaluated) and b western blot analysis on total protein extracts from Deltasua5 yeast cells expressing human YRDC variants using anti-hYRDC antibody. c , d Mass spectrometry (LC-MS/MS) quantification of t 6 A modification in total tRNAs extracted from Deltasua5 yeast cells expressing human YRDC variants ( c ) (mean +- s.e.m. of two independent LC-MS/MS experiments (technical replicates), each measuring samples from three independent yeast transformants; one-way ANOVA ( F (5,28) = 269.4, p < 0.0001), Dunnett's multiple comparisons test, *** p = 0.0008, **** p < 0.0001) and from cultured primary skin fibroblasts from controls (two unaffected individuals) and affected individuals with either the p.Tyr7* GON7 mutation (two individuals), or the YRDC mutations (three individuals) or with the p.Arg352Gln OSGEP mutation in the homozygous state (individual < > described in Braun et al. 13 ) ( d ) (mean +- s.e.m. of two independent LC-MS/MS experiments (technical replicates), each measuring samples from three independent cell culture experiments; one-way ANOVA ( F (3,44) = 6.446, p < 0.001), Dunnett's multiple comparisons test, n.s. = 0.4894, * p = 0.0169, *** p = 0.008). Source data are provided as a Source Data file