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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- 657602 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#657602, RRID:AB_2562631
- Product name
- Purified anti-IRF1
- Antibody type
- Monoclonal
- Antigen
- Partial human IRF1 recombinant protein (147-325 a.a.)
- Description
- IRF1 is the first identified member of the interferon regulatory transcription factor family. Its expression is induced in response to pathogen infection or the stimulation of several cytokines, including type I and II IFN, IL-1, IL-6, and TNF-α. Upon stimulation, IRF1 is transcriptionally activated by STAT1 and NFκB transcription factors via the GAS (interferon-gamma-activated sequence) element in the IRF1 promoter. IRF1 plays important roles in mediating immune response during infection and development of T cells and NK cells. In addition to the immunomodulatory functions, IRF1 has also shown to be involved in the regulation of cell cycle progression, tumor suppression, and apoptosis. IRF1-mediated transcription can be repressed by IRF2 through competing for the same binding region on the promoters.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 13H3A44
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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- BioLegend (provider)
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- Experimental details
- THP-1 cells were stained with purified anti-IRF1 (clone 13H3A44) antibody, followed by staining with DyLight™ 488 conjugated goat anti-mouse IgG (green) antibody. Nuclei were stained with DAPI (blue).
- Conjugate
- Unconjugated
