11335-1-AP

antibody from Proteintech Group

Targeting: IRF1 MAR

Provider product page for 11335-1-AP

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ELISA

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Flow cytometry

Antibody data

Antibody Data
Antigen structure
References [59]
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Western blot [1]

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Product number: 11335-1-AP - Provider product page
Provider: Proteintech Group
Product name: IRF1 antibody
Antibody type: Polyclonal
Description: KD/KO validated IRF1 antibody (Cat. #11335-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IHC, IP, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

IRF1 protein structure - 11335-1-AP shown in red.

Submitted references Inflammatory signaling differentially changes chromatin accessibility and gene expression of the PD- associated kinase LRRK2 between human and mice.

Beilina A, Park JH, Landeck N, Chia R, Ding J, Reed X, Bailey H, Kim C, Kaganovich A, Acri DJ, Brooks J, Mann A, Zaghloul KA, Marsan E, Raphael Gibbs J, DeCasien A, Cookson MR
Molecular neurodegeneration 2026 Mar 18;21(1)

Inflammatory signaling differentially changes chromatin accessibility and gene expression of the PD- associated kinase LRRK2 between human and mice.

Beilina A, Park JH, Landeck N, Chia R, Ding J, Reed X, Bailey H, Kim C, Kaganovich A, Acri DJ, Brooks J, Mann A, Zaghloul KA, Marsan E, Gibbs JR, DeCasien A, Cookson MR
bioRxiv : the preprint server for biology 2026 Jan 12;

Luteolin ameliorates steroid-induced osteonecrosis of the femoral head via a gut microbiota-L-Carnitine-IFIH1 axis.

Li T, Li Y, Gao J, Ma R, Peng Y, Zhang Q, Chen Y, Wang W
Journal of translational medicine 2026 Feb 6;24(1)

Tumor-Intrinsic ARHGEF3 Enhances Antitumor Immunity by Promoting T-Cell Infiltration and Limiting Myeloid Cell-Mediated Immunosuppression.

Li Y, Wang L, Zhang Z, Qian C, Li N, Huang W, Ba Q, Liu X, Sun M
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2026 Apr 23;:e23895

Reciprocal activation between M1 macrophages and trophoblasts through CXCL9/STAT1/ZEB1/CCL2 axis promotes recurrent spontaneous abortion.

Yan S, Wang X, Wu Q, Ding J, Qiu H
Frontiers in immunology 2025;16:1629370

Coptisine Improves Liver Inflammation in Sepsis by Regulating STAT1/IRF1/GPX4 Signaling-Mediated Kupffer Cells Ferroptosis.

Zhu B, Sun C, Luo D, Liang Y, Jiang A, Jiang Z, Luo H, Yuan G, Huang C, Liu S, Li L, Jiang Y
Phytotherapy research : PTR 2025 Sep;39(9):4308-4326

Stromal Stiffness-Regulated IGF2BP2 in Pancreatic Cancer Drives Immune Evasion via Sphingomyelin Metabolism.

Tang R, Zhang Z, Liu X, Liao Y, Chen Y, Xiao M, Li Y, Zhou C, Tan Z, Zhang C, Chen C, Rong Z, Liu Y, Li P, Du Q, He Q, Lei Y, Wu Z, Lu S, Xu J, Wang W, Shi S, Yu X
Gastroenterology 2025 Sep;169(4):615-631.e32

SMARCA4 regulates inducible BRD4 genomic redistribution coupling intrinsic immunity and plasticity in epithelial injury-repair.

Xu X, Brasier AR
Nucleic acids research 2025 Mar 20;53(6)

Gut microbial metabolite 4-hydroxybenzeneacetic acid drives colorectal cancer progression via accumulation of immunosuppressive PMN-MDSCs.

Liao Q, Zhou X, Wu L, Yang Y, Zhu X, Liao H, Zhang Y, Lian W, Zhang F, Wang H, Ding Y, Zhao L
The Journal of clinical investigation 2025 Jun 2;135(11)

Celastrol enhanced CD8+T cell immunity in melanoma by targeting SHP2 and upregulating MHC-I.

Kong Q, Liu S, He S, Luo Z, Lei R, Wang R, Liu X, Wu J
Phytomedicine : international journal of phytotherapy and phytopharmacology 2025 Jul;142:156731

Luminal hormone-responsive cells tune the regenerative remodeling of mammary glands in large mammals.

Li Y, Zhang L, Luo T, Zhang W, Wang T, Liu F, Lin S, Luo J, Liu J, Peng J, Wang C, Wang W, Shi H
Cell discovery 2025 Dec 30;11(1):105

The MLL3/GRHL2 complex regulates malignant transformation and anti-tumor immunity in squamous cancer.

Nam C, Huang G, Zheng Y, Zhao H, Pan Y, Hu B, Wenger T, Van HT, Xu LY, Li EM, Koeffler HP, Ge K, Dou Y, Sinha UK, Park YM, Lin DC
The Journal of experimental medicine 2025 Apr 7;222(4)

LncRNA EMBP1 sponges miR-454-3p to upregulate IRF1 and activate NLRP3-mediated chondrocyte pyroptosis to drive osteoarthritis progression.

Ma S, Yu P, Ma J, Liu K, Wang M, Shi P, Duong NTD, Cheng S, Wang S
International immunopharmacology 2025 Apr 24;153:114460

PLA2G7 promotes immune evasion of bladder cancer through the JAK-STAT-PDL1 axis.

Peng D, Yang W, Tang T, He A, Xu X, Jing T, Xia D
Cell death & disease 2025 Apr 1;16(1):234

Cooperative interaction of interferon regulatory factor -1 and bromodomain-containing protein 4 on RNA polymerase activation for intrinsic innate immunity.

Xu X, Qiao D, Brasier AR
Frontiers in immunology 2024;15:1366235

IRF1 and IL1A associated with PANoptosis serve as potential immune signatures for lung ischemia reperfusion injury following lung transplantation.

Zhang N, Zhang Q, Zhang Z, Yu J, Fu Y, Gao J, Jiang X, Jiang P, Wen Z
International immunopharmacology 2024 Sep 30;139:112739

Sulforaphane improves post-resuscitation myocardial dysfunction by inhibiting cardiomyocytes ferroptosis via the Nrf2/IRF1/GPX4 pathway.

Zheng Z, Xu J, Mao Y, Mei Z, Zhu J, Lan P, Wu X, Xu S, Zhang M
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2024 Oct;179:117408

IRF1-mediated upregulation of PARP12 promotes cartilage degradation by inhibiting PINK1/Parkin dependent mitophagy through ISG15 attenuating ubiquitylation and SUMOylation of MFN1/2.

Deng Z, Long D, Li C, Liu H, Li W, Zhong Y, Mo X, Li R, Yang Z, Kang Y, Mao G
Bone research 2024 Oct 28;12(1):63

Nur77-IRF1 axis inhibits esophageal squamous cell carcinoma growth and improves anti-PD-1 treatment efficacy.

Shi H, Chen L, Wang T, Zhang W, Liu J, Huang Y, Li J, Qi H, Wu Z, Wang Y, Chen H, Zhu Y, Li Q
Cell death discovery 2024 May 24;10(1):254

Transcriptomics confirms IRF1 as a key regulator of pyroptosis in diabetic retinopathy.

Xian Y, Wang X, Yu Y, Chen X
Biochemical and biophysical research communications 2024 May 21;709:149760

Stenotrophomonas maltophilia contributes to smoking-related emphysema through IRF1-triggered PANoptosis of alveolar epithelial cells.

Xia H, Lin J, Wang Y, Yu J, Wang H, Cheng C, Yang Y, Bian T, Wu Y, Liu Q
Environmental pollution (Barking, Essex : 1987) 2024 May 15;349:123913

Targeting autophagy overcomes cancer-intrinsic resistance to CAR-T immunotherapy in B-cell malignancies.

Tang L, Zhang H, Zhou F, Wei Q, Du M, Wu J, Li C, Luo W, Zhou J, Wang X, Chen Z, Zhang Y, Huang Z, Wu Z, Wen Y, Jiang H, Liao D, Kou H, Xiong W, Mei H, Hu Y
Cancer communications (London, England) 2024 Mar;44(3):408-432

Interferon-γ in the tumor microenvironment promotes the expression of B7H4 in colorectal cancer cells, thereby inhibiting cytotoxic T cells.

Jing ZL, Liu GL, Zhou N, Xu DY, Feng N, Lei Y, Ma LL, Tang MS, Tong GH, Tang N, Deng YJ
Scientific reports 2024 Mar 13;14(1):6053

Mefloquine enhances the efficacy of anti-PD-1 immunotherapy via IFN-γ-STAT1-IRF1-LPCAT3-induced ferroptosis in tumors.

Tao Q, Liu N, Wu J, Chen J, Chen X, Peng C
Journal for immunotherapy of cancer 2024 Mar 11;12(3)

Role of deubiquitinase USP47 in cardiac function alleviation and anti-inflammatory immunity after myocardial infarction by regulating NLRP3 inflammasome-mediated pyroptotic signal pathways.

Wu Z, Li W, Wang S, Zheng Z
International immunopharmacology 2024 Jul 30;136:112346

Identification and experimental verification of immune-related hub genes in intervertebral disc degeneration.

Huang Z, Cai X, Shen X, Chen Z, Zhang Q, Liu Y, Lu B, Xu B, Li Y
Heliyon 2024 Jul 30;10(14):e34530

IRF1 regulation of ZBP1 links mitochondrial DNA and chondrocyte damage in osteoarthritis.

Sun K, Lu F, Hou L, Zhang X, Pan C, Liu H, Zheng Z, Guo Z, Ruan Z, Hou Y, Zhang J, Guo F, Zhu W
Cell communication and signaling : CCS 2024 Jul 18;22(1):366

BATF2 is a regulator of interferon-γ signaling in astrocytes during neuroinflammation.

Tinkey RA, Smith BC, Habean ML, Williams JL
bioRxiv : the preprint server for biology 2024 Jul 16;

MICA+ Tumor Cell Upregulated Macrophage-Secreted MMP9 via PROS1-AXL Axis to Induce Tumor Immune Escape in Advanced Hepatocellular Carcinoma (HCC).

Wu Q, Li X, Yang Y, Huang J, Yao M, Li J, Huang Y, Cai X, Geller DA, Yan Y
Cancers 2024 Jan 8;16(2)

Targeting the immune privilege of tumor-initiating cells to enhance cancer immunotherapy.

Yang C, Geng H, Yang X, Ji S, Liu Z, Feng H, Li Q, Zhang T, Zhang S, Ma X, Zhu C, Xu N, Xia Y, Li Y, Wang H, Yu C, Du S, Miao B, Xu L, Wang H, Cao Y, Li B, Zhu L, Tang X, Zhang H, Zhu C, Huang Z, Leng C, Hu H, Chen X, Yuan S, Jin G, Bernards R, Sun C, Zheng Q, Qin W, Gao Q, Wang C
Cancer cell 2024 Dec 9;42(12):2064-2081.e19

Integrating bioinformatics and experimental validation to Investigate IRF1 as a novel biomarker for nucleus pulposus cells necroptosis in intervertebral disc degeneration.

Zhou K, Wu S, Wu Z, Ran R, Song W, Dong H, Zhang H
Scientific reports 2024 Dec 3;14(1):30138

Type I/type III IFN and related factors regulate JEV infection and BBB endothelial integrity.

Zhang YG, Zhang HX, Chen HW, Lv P, Su J, Chen YR, Fu ZF, Cui M
Journal of neuroinflammation 2023 Sep 27;20(1):216

IRF1 suppresses colon cancer proliferation by reducing SPI1-mediated transcriptional activation of GPX4 and promoting ferroptosis.

Chen Y, Lin B, Yang S, Huang J
Experimental cell research 2023 Oct 1;431(1):113733

Characterization of the induction kinetics and antiviral functions of IRF1, ISG15 and ISG20 in cells infected with gammacoronavirus avian infectious bronchitis virus.

Liu SY, Huang M, Fung TS, Chen RA, Liu DX
Virology 2023 May;582:114-127

Chlorogenic Acid Alleviates Hepatic Ischemia-Reperfusion Injury by Inhibiting Oxidative Stress, Inflammation, and Mitochondria-Mediated Apoptosis In Vivo and In Vitro.

Li K, Feng Z, Wang L, Ma X, Wang L, Liu K, Geng X, Peng C
Inflammation 2023 Jun;46(3):1061-1076

Human IRF1 governs macrophagic IFN-γ immunity to mycobacteria.

Rosain J, Neehus AL, Manry J, Yang R, Le Pen J, Daher W, Liu Z, Chan YH, Tahuil N, Türel Ö, Bourgey M, Ogishi M, Doisne JM, Izquierdo HM, Shirasaki T, Le Voyer T, Guérin A, Bastard P, Moncada-Vélez M, Han JE, Khan T, Rapaport F, Hong SH, Cheung A, Haake K, Mindt BC, Pérez L, Philippot Q, Lee D, Zhang P, Rinchai D, Al Ali F, Ahmad Ata MM, Rahman M, Peel JN, Heissel S, Molina H, Kendir-Demirkol Y, Bailey R, Zhao S, Bohlen J, Mancini M, Seeleuthner Y, Roelens M, Lorenzo L, Soudée C, Paz MEJ, González ML, Jeljeli M, Soulier J, Romana S, L'Honneur AS, Materna M, Martínez-Barricarte R, Pochon M, Oleaga-Quintas C, Michev A, Migaud M, Lévy R, Alyanakian MA, Rozenberg F, Croft CA, Vogt G, Emile JF, Kremer L, Ma CS, Fritz JH, Lemon SM, Spaan AN, Manel N, Abel L, MacDonald MR, Boisson-Dupuis S, Marr N, Tangye SG, Di Santo JP, Zhang Q, Zhang SY, Rice CM, Béziat V, Lachmann N, Langlais D, Casanova JL, Gros P, Bustamante J
Cell 2023 Feb 2;186(3):621-645.e33

Comprehensive Analysis of PANoptosis-Related Gene Signature of Ulcerative Colitis.

Wang JM, Yang J, Xia WY, Wang YM, Zhu YB, Huang Q, Feng T, Xie LS, Li SH, Liu SQ, Yu SG, Wu QF
International journal of molecular sciences 2023 Dec 26;25(1)

IFNγ Transcribed by IRF1 in CD4+ Effector Memory T Cells Promotes Senescence-Associated Pulmonary Fibrosis.

Chen H, Wang Q, Li J, Li Y, Chen A, Zhou J, Zhao J, Mao Z, Zhou Z, Zhang J, Wang Y, Wang R, Li Q, Zhang Y, Jiang R, Miao D, Jin J
Aging and disease 2023 Dec 1;14(6):2215-2237

Targeting ULK1 Decreases IFNγ-Mediated Resistance to Immune Checkpoint Inhibitors.

Fenton SE, Zannikou M, Ilut L, Fischietti M, Ji C, Oku CV, Horvath CM, Le Poole IC, Bosenberg M, Bartom ET, Kocherginsky M, Platanias LC, Saleiro D
Molecular cancer research : MCR 2023 Apr 1;21(4):332-344

Toosendanin suppresses African swine fever virus replication through upregulating interferon regulatory factor 1 in porcine alveolar macrophage cultures.

Liu Y, Zhang X, Liu Z, Huang L, Jia W, Lian X, Weng C, Zhang G, Qi W, Chen J
Frontiers in microbiology 2022;13:970501

IRF1/ZNF350/GPX4-mediated ferroptosis of renal tubular epithelial cells promote chronic renal allograft interstitial fibrosis.

Zhang Y, Zhang J, Feng D, Zhou H, Gui Z, Zheng M, Hang Z, Wang Z, Wang Z, Gu M, Tan R
Free radical biology & medicine 2022 Nov 20;193(Pt 2):579-594

Nanoparticle delivery of miR-21-3p sensitizes melanoma to anti-PD-1 immunotherapy by promoting ferroptosis.

Guo W, Wu Z, Chen J, Guo S, You W, Wang S, Ma J, Wang H, Wang X, Wang H, Ma J, Yang Y, Tian Y, Shi Q, Gao T, Yi X, Li C
Journal for immunotherapy of cancer 2022 Jun;10(6)

Human lung adenocarcinoma CD47 is upregulated by interferon-γ and promotes tumor metastasis.

Qu S, Jiao Z, Lu G, Xu J, Yao B, Wang T, Wang J, Yao Y, Yan X, Wang T, Liang H, Zen K
Molecular therapy oncolytics 2022 Jun 16;25:276-287

Huganbuzure Granule Attenuates Concanavalin-A-Induced Immune Liver Injury in Mice via Regulating the Balance of Th1/Th2/Th17/Treg Cells and Inhibiting Apoptosis.

Wang M, Yin H, Xia Y, Tu Y, Zou X, Song W, Luo L, Wu H, Yang Y, Zan J, Liu Y, Dan H, Yin Q, You P
Evidence-based complementary and alternative medicine : eCAM 2021;2021:5578021

MAGE-C3 promotes cancer metastasis by inducing epithelial-mesenchymal transition and immunosuppression in esophageal squamous cell carcinoma.

Wu Q, Zhang W, Wang Y, Min Q, Zhang H, Dong D, Zhan Q
Cancer communications (London, England) 2021 Dec;41(12):1354-1372

Anti-Inflammatory and Antioxidant Effect of Eucommia ulmoides Polysaccharide in Hepatic Ischemia-Reperfusion Injury by Regulating ROS and the TLR-4-NF-κB Pathway.

Gao W, Feng Z, Zhang S, Wu B, Geng X, Fan G, Duan Y, Li K, Liu K, Peng C
BioMed research international 2020;2020:1860637

Amelogenin Downregulates Interferon Gamma-Induced Major Histocompatibility Complex Class II Expression Through Suppression of Euchromatin Formation in the Class II Transactivator Promoter IV Region in Macrophages.

Yotsumoto K, Sanui T, Tanaka U, Yamato H, Alshargabi R, Shinjo T, Nakao Y, Watanabe Y, Hayashi C, Taketomi T, Fukuda T, Nishimura F
Frontiers in immunology 2020;11:709

Aucubin Attenuates Liver Ischemia-Reperfusion Injury by Inhibiting the HMGB1/TLR-4/NF-κB Signaling Pathway, Oxidative Stress, and Apoptosis.

Zhang S, Feng Z, Gao W, Duan Y, Fan G, Geng X, Wu B, Li K, Liu K, Peng C
Frontiers in pharmacology 2020;11:544124

m(6) A RNA methyltransferases METTL3/14 regulate immune responses to anti-PD-1 therapy.

Wang L, Hui H, Agrawal K, Kang Y, Li N, Tang R, Yuan J, Rana TM
The EMBO journal 2020 Oct 15;39(20):e104514

DcR3 promotes proliferation and invasion of pancreatic cancer via a DcR3/STAT1/IRF1 feedback loop.

Wei Y, Chen X, Yang J, Yao J, Yin N, Zhang Z, Li D, Zhu D, Zhou J
American journal of cancer research 2019;9(12):2618-2633

IRF1 inhibits the proliferation and metastasis of colorectal cancer by suppressing the RAS-RAC1 pathway.

Hong M, Zhang Z, Chen Q, Lu Y, Zhang J, Lin C, Zhang F, Zhang W, Li X, Zhang W, Li X
Cancer management and research 2019;11:369-378

Interferon gamma inhibits transmissible gastroenteritis virus infection mediated by an IRF1 signaling pathway.

Shan L, Fu F, Xue M, Zhu X, Li L, Feng L, Liu P
Archives of virology 2019 Nov;164(11):2659-2669

The RNA N(6)-methyladenosine modification landscape of human fetal tissues.

Xiao S, Cao S, Huang Q, Xia L, Deng M, Yang M, Jia G, Liu X, Shi J, Wang W, Li Y, Liu S, Zhu H, Tan K, Luo Q, Zhong M, He C, Xia L
Nature cell biology 2019 May;21(5):651-661

Pioglitazone decreased renal calcium oxalate crystal formation by suppressing M1 macrophage polarization via the PPAR-γ-miR-23 axis.

Chen Z, Yuan P, Sun X, Tang K, Liu H, Han S, Ye T, Liu X, Yang X, Zeng J, Yan L, Xing J, Xiao K, Ye Z, Xu H
American journal of physiology. Renal physiology 2019 Jul 1;317(1):F137-F151

Glycyrrhizic acid ammonium salt alleviates Concanavalin A-induced immunological liver injury in mice through the regulation of the balance of immune cells and the inhibition of hepatocyte apoptosis.

Tian X, Liu Y, Liu X, Gao S, Sun X
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2019 Dec;120:109481

Toxoplasma Effector TgIST Targets Host IDO1 to Antagonize the IFN-γ-Induced Anti-parasitic Response in Human Cells.

Bando H, Sakaguchi N, Lee Y, Pradipta A, Ma JS, Tanaka S, Lai DH, Liu J, Lun ZR, Nishikawa Y, Sasai M, Yamamoto M
Frontiers in immunology 2018;9:2073

IRF1 up-regulates isg15 gene expression in dsRNA stimulation or CSFV infection by targeting nucleotides -487 to -325 in the 5' flanking region.

Li XQ, Li XN, Liang JJ, Cai XB, Tao Q, Li YX, Qin Q, Xu SP, Luo TR
Molecular immunology 2018 Feb;94:153-165

Porphyromonas gingivalis and its lipopolysaccharide differently modulate epidermal growth factor-dependent signaling in human gingival epithelial cells.

Elkaim R, Bugueno-Valdebenito IM, Benkirane-Jessel N, Tenenbaum H
Journal of oral microbiology 2017;9(1):1334503

IFN-γ-mediated IRF1/miR-29b feedback loop suppresses colorectal cancer cell growth and metastasis by repressing IGF1.

Yuan L, Zhou C, Lu Y, Hong M, Zhang Z, Zhang Z, Chang Y, Zhang C, Li X
Cancer letters 2015 Apr 1;359(1):136-47

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The IRF1 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human IRF1. This antibody recognizes human,mouse,rat antigen. The IRF1 antibody has been validated for the following applications: ELISA, WB analysis.
Appendix: 11335-1-AP.pdf

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