Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-50512 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IRF1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- The antibody detects endogenous levels of total IRF1 protein.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.5 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Irf1 was performed by loading (from left to right): NIH/3T3 and Jurkat cell lysates (30µg) on to a 12% SDS-PAGE gel. Proteins were transferred to a membrane and the membrane was probed with a Irf1 antibody (Product # PA5-50512) at a 1/750 dilution for 1 minute, followed by a secondary antibody at 1/8000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of IRF1 in paraffin embedded Human liver cancer tissue using IRF1 Polyclonal Antibody (Product # PA5-50512) at a 1:40 dilution.