Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
- Flow cytometry [1]
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Validation data
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- Product number
- MA5-31996 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IRF1 Recombinant Rabbit Monoclonal Antibody (SR44-08)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- SR44-08
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IRF1 in different lysates using a Monoclonal antibody (Product #MA5-31996) at a dilution of 1:1,000. Positive control: Lane 1: PC-12, Lane 2: Jurkat.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IRF1 in different lysates using a Monoclonal antibody (Product #MA5-31996) at a dilution of 1:1,000. Positive control: Lane 1: PC-12, Lane 2: Jurkat.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-IRF1 Recombinant Rabbit Monoclonal Antibody (SR44-08) (Product # MA5-31996) and a band at ~45 kDa corresponding to IRF1 was induced upon IFN gamma treatment in HeLa and A549. An uncharacterized band (*) at ~35 kDa was observed across the samples tested. Modified whole cell extracts (30 µg lysate) of HeLa (Lane 1), HeLa treated with IFN gamma (100ng/ml, 4hr) (Lane 2) and A549 (Lane 3), A549 treated with IFN gamma (100ng/ml, 4hr) and Raji (Lane 5) were electrophoresed using Novex® NuPAGE® 4-12% % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005)..
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of IRF1 in NIH/3T3 cells using a IRF1 Monoclonal antibody (Product # MA5-31996) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of IRF1 in Hela cells using a IRF1 Monoclonal antibody (Product # MA5-31996) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of IRF1 of paraffin-embedded Human colon cancer tissue using a IRF1 Monoclonal antibody (Product #MA5-31996). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of IRF1 of paraffin-embedded Mouse brain tissue using a IRF1 Monoclonal antibody (Product #MA5-31996). Counter stained with hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometric analysis of IRF1 in Jurkat cells using a IRF1 Monoclonal Antibody (Product # MA5-31996) at a dilution of 1:50, as seen in blue compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.