Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- AF3057 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse FGF-21 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse FGF-21 in direct ELISAs and Western blots. In these formats, approximately 15% cross-reactivity with recombinant human (rh) FGF-21 is observed and less than 1% cross-reactivity with rhFGF-19 and rhFGF-23 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q9JJN1
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Chronic Over-expression of Fibroblast Growth Factor 21 Increases Bile Acid Biosynthesis by Opposing FGF15/19 Action.
Activation of AMPKα2 is not crucial for mitochondrial uncoupling-induced metabolic effects but required to maintain skeletal muscle integrity.
Silencing of the Fibroblast growth factor 21 gene is an underlying cause of acinar cell injury in mice lacking MIST1.
Activation of the farnesoid X receptor induces hepatic expression and secretion of fibroblast growth factor 21.
BetaKlotho is required for metabolic activity of fibroblast growth factor 21.
Zhang J, Gupte J, Gong Y, Weiszmann J, Zhang Y, Lee KJ, Richards WG, Li Y
EBioMedicine 2017 Feb;15:173-183
EBioMedicine 2017 Feb;15:173-183
Activation of AMPKα2 is not crucial for mitochondrial uncoupling-induced metabolic effects but required to maintain skeletal muscle integrity.
Ost M, Werner F, Dokas J, Klaus S, Voigt A
PloS one 2014;9(4):e94689
PloS one 2014;9(4):e94689
Silencing of the Fibroblast growth factor 21 gene is an underlying cause of acinar cell injury in mice lacking MIST1.
Johnson CL, Mehmood R, Laing SW, Stepniak CV, Kharitonenkov A, Pin CL
American journal of physiology. Endocrinology and metabolism 2014 Apr 15;306(8):E916-28
American journal of physiology. Endocrinology and metabolism 2014 Apr 15;306(8):E916-28
Activation of the farnesoid X receptor induces hepatic expression and secretion of fibroblast growth factor 21.
Cyphert HA, Ge X, Kohan AB, Salati LM, Zhang Y, Hillgartner FB
The Journal of biological chemistry 2012 Jul 20;287(30):25123-38
The Journal of biological chemistry 2012 Jul 20;287(30):25123-38
BetaKlotho is required for metabolic activity of fibroblast growth factor 21.
Ogawa Y, Kurosu H, Yamamoto M, Nandi A, Rosenblatt KP, Goetz R, Eliseenkova AV, Mohammadi M, Kuro-o M
Proceedings of the National Academy of Sciences of the United States of America 2007 May 1;104(18):7432-7
Proceedings of the National Academy of Sciences of the United States of America 2007 May 1;104(18):7432-7
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse FGF-21 by Western Blot. Western blot shows lysates of mouse spleen tissue and rat spleen tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse FGF-21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3057) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for FGF-21 at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.