NBP2-00645

antibody from Novus Biologicals

Targeting: FGF21

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-00645

Provider

Novus Biologicals

Product name

Mouse Monoclonal FGF-21 Antibody

Antibody type

Monoclonal

Description

Affinity purified.

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

0.1 ml

Concentration

1.4 mg/ml

Storage

Store at -20C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: FGF21 Antibody (2F10) [NBP2-00645] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FGF21 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FGF21.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: FGF21 Antibody (2F10) [NBP2-00645] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-FGF21 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: FGF21 Antibody (2F10) [NBP2-00645] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-FGF21 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: FGF21 Antibody (2F10) [NBP2-00645] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-FGF21 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: FGF21 Antibody (2F10) [NBP2-00645] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-FGF21 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: FGF21 Antibody (2F10) [NBP2-00645] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-FGF21 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: FGF21 Antibody (2F10) [NBP2-00645] - Staining of paraffin-embedded Human breast tissue using anti-FGF21 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: FGF21 Antibody (2F10) [NBP2-00645] - Staining of paraffin-embedded Human pancreas tissue using anti-FGF21 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: FGF21 Antibody (2F10) [NBP2-00645] Staining of paraffin-embedded Human tonsil using anti-FGF21 mouse monoclonal antibody.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: FGF21 Antibody (2F10) [NBP2-00645] - Analysis of Jurkat cells, using anti-FGF21 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: FGF-21 Antibody (2F10) [NBP2-00645] - HEK293T cells transfected with either RC204538 overexpress plasmid (Red) or empty vector control plasmid(Blue) were immunostained by anti-FGF21 antibody and then analyzed by flow cytometry.