V9462

antibody from NSJ Bioreagents

Targeting: FGF21

Immunohistochemistry (Supportive data in Antibodypedia)

Protein array (Supportive data in Antibodypedia)

Antibody Data

Product number

V9462

Provider

NSJ Bioreagents

Product name

Fibroblast Growth Factor 21 Antibody / FGF21

Antibody type

Monoclonal

Description

This highly specific Fibroblast Growth Factor 21 antibody is suitable for use in Immunohistochemistry applications with human samples.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

FGF21/4343

Vial size

20 ug (with BSA and sodium azide), 100 ug (with BSA and sodium azide), 100 ug (without BSA or sodium azide), 7 ml IHC only format (if applicable)

Concentration

0.2 mg/ml, 1 mg/ml

Storage

Aliquot the Fibroblast Growth Factor 21 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human lung tissue with Fibroblast Growth Factor 21 antibody (clone FGF21/4343). Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Protein array

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Fibroblast Growth Factor 21 antibody (clone FGF21/4343). These results demonstrate the foremost specificity of the FGF21/4343 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.