MA5-11140

antibody from Invitrogen Antibodies

Targeting: KMT5A PR-Set7, SET07, SET8, SETD8

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-11140

Provider

Invitrogen Antibodies

Product name

SETD8 Monoclonal Antibody (43AT551.86)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

43AT551.86

Vial size

400 µL

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SETD8 in various lysates. Samples were incubated with SETD8 monoclonal antibody (Product # MA5-11140) using a dilution of 1:2,000 followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a dilution of 1:10,000. Lysates/proteins: 20 µg per lane. Lane 1: 293T whole cell lysate; Lane 2: 293 whole cell lysate; Lane 3: K562 whole cell lysate. Predicted band size: 43 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HeLa cells using a SET07 monoclonal antibody (Product # MA5-11140) at a dilution of 0.025 mg/mL and followed by PE-conjugated goat anti-mouse lgG secondary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of SETD8 in HeLa cells. Samples were incubated with SETD8 monoclonal antibody (Product # MA5-11140) using a dilution of 0.025 mg/mL followed by PE-conjugated goat anti-mouse lgG (whole molecule). (Top) Negative control of Hela cells without PE-conjugated goat anti-mouse lgG (whole molecule). PE-conjugated goat anti-mouse lgG emits red fluorescence. (Bottom) Immunofluorescence analysis of SET07 Antibody in HeLa cells.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of SETD8 in formalin-fixed and paraffin-embedded human testis tissue. Samples were incubated with SETD8 monoclonal antibody (Product # MA5-11140) which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.