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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- STJ99266 - Provider product page
- Provider
- St John's Laboratory
- Product name
- Anti-EIF2S1 antibody [2E4-F5-E7] (STJ99266)
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody anti-Eukaryotic Translation Initiation Factor 2 Subunit 1 is suitable for use in Western Blot, Immunofluorescence, Immunocytochemistry and Immunoprecipitation research applications.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
NA- Epitope
- NA
- Isotype
- IgG
- Antibody clone number
- NA
- Vial size
- NA
- Concentration
- NA
- Storage
- Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
- Handling
- NA
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Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Western blot detection of eIF2 Alpha in K562, COS7, 3T3, C2C12, MCF-7, C6 and Hela cell lysates using eIF2 Alpha mouse mAb (1:1000 diluted).Predicted band size:38KDa.Observed band size:38KDa.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Hela cells fixed by anhydrous methanol for 2 h at-20℃ and using anti-eIF2 Alpha mouse mAb (dilution 1:200).
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of Hela cell lysates using eIF2 Alpha.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
