Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [1]
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Validation data
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- Product number
- LS-C808608 - Provider product page
- Provider
- LSBio
- Product name
- EIF2S1 Antibody LS-C808608
- Antibody type
- Polyclonal
- Description
- Peptide affinity chromatography.
- Reactivity
- Human, Mouse, Rat, Porcine
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- MCF-7 and MDA-MB-231 cells were treated with the indicated concentrations of BA for 24h, and the protein levels of ER stress-associated signals were stimulated by BA in a dose-dependent manner, including GRP78, p-PERK/PERK, p-eIF2a/eIF2a, CHOP, and caspase-12.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Western blot analysis of eIF2 alpha expression in IFN-a treated K562 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- MCF-7 and MDA-MB-231 cells were treated with the indicated concentrations of BA for 24h, and the protein levels of ER stress-associated signals were stimulated by BA in a dose-dependent manner, including GRP78, p-PERK/PERK, p-eIF2a/eIF2a, CHOP, and caspase-12.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Western blot analysis of eIF2 alpha expression in IFN-a treated K562 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- 1:100 staining human liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.