16417-1-AP

antibody from Proteintech Group

Targeting: BCAT2 BCAM, BCT2

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

16417-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#16417-1-AP, RRID:AB_10792411

Product name

BCAT2 antibody

Antibody type

Polyclonal

Description

KD/KO validated BCAT2 antibody (Cat. #16417-1-AP) is a rabbit polyclonal antibody that shows reactivity with Human and has been validated for the following applications: IHC, WB,ELISA.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Vascular Smooth Muscle Cell-Specific BCAT2 Deficiency Attenuates Diabetic Atherosclerotic Calcification via Histone Propionylation.

Zhang L, Yang Y, Yuan W, Dai Z, Li L, Sun Z, Shao C, Zang G, Qian Y, Wang Z
Research (Washington, D.C.) 2026;9:1052

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ZNF33A activates Wnt/β-catenin signaling and promotes BCAA catabolism in colorectal cancer.

Yu W, Chen J, Pu X, Shen H, Cao L, Zhu L, Pei H, Lin X
Cell communication and signaling : CCS 2026 Mar 9;24(1)

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HucMSC-sEV-derived miR-320d alleviates IBD by inhibiting macrophage ferroptosis through m6A-mediated BCAT2 upregulation.

Wang N, Yuan J, Wang X, Yao D, Ocansey DKW, Mao F
Bioorganic chemistry 2026 Jul 5;175:109817

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Alpha-ketoglutarate supplementation improves hyperglycemia and attenuates the decrease in GLUT4 and PGC-1α proteins in adipose tissue of streptozotocin-high-fat diet-induced diabetic mice.

Takemura A, Matsunaga Y, Hajime S, Wang W, Takahashi Y, Hatta H
Journal of nutritional science 2025;14:e89

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Impaired xCT-mediated cystine uptake drives serine and proline metabolic reprogramming and mitochondrial fission in skeletal muscle cells.

Kanaan MN, Karam CY, Kennedy LS, Pileggi CA, Hamilton L, Cuperlovic-Culf M, Harper ME
Redox biology 2025 Oct;86:103839

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Ginsenoside Ro Targets BCAT1/2 to Suppress Ferroptosis: A Novel Therapeutic Strategy for Ischemic Cardioprotection.

Liu J, Wang F, Bai J, Qu S, Tao S, Liu C, Wang Q, Ding W, Lai Q, Zhang J
Journal of agricultural and food chemistry 2025 Oct 22;73(42):26683-26699

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BCAA exaggerated acute and chronic ischemic heart disease through promotion of NLRP3 via Sirt1.

Peng Y, Duan Y, Wang D, Liu J, Zhao G, Ji Z, Zhang J, Wei B
Biochemical pharmacology 2025 Nov;241:117150

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Modified Xiaoyaosan rescues depression-like behavior via remodeling gut microbiota and leucine metabolism.

Zhao Y, Wang Q, Wu Z, Zhou Y, Gao X, Gong W, Qin X, Ren Y, Tian J
Phytomedicine : international journal of phytotherapy and phytopharmacology 2025 Nov;147:157241

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A nuclear branched-chain amino acid catabolism pathway controls histone propionylation in pancreatic cancer.

Demetriadou C, Noji M, Good AL, Mitchell-Velasquez E, Venkatesh S, Kantner DS, Pennise J, Costa-Pinheiro P, Pinheiro LV, Harada T, Nguyen PTT, Chatoff A, Megill E, Crispim CVDS, Marcinkiewicz MM, Meier JL, Arany Z, Asangani I, Furth EE, Stanger BZ, Snyder NW, Wellen KE
bioRxiv : the preprint server for biology 2025 Apr 26;

---

KIC (ketoisocaproic acid) and leucine have divergent effects on tissue insulin signaling but not on whole-body insulin sensitivity in rats.

Mann G, Mora S, Adegoke OAJ
PloS one 2024;19(8):e0309324

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Branched-chain amino acids deficiency promotes diabetic cardiomyopathy by activating autophagy of cardiac fibroblasts.

Zhou ZY, Song K, Liu ZY, Ke YF, Shi Y, Cai K, Zhao R, Sun X, Tao H, Zhao JY
Theranostics 2024;14(19):7333-7348

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Elevated BCAA catabolism reverses the effect of branched-chain ketoacids on glucose transport in mTORC1-dependent manner in L6 myotubes.

Mann G, Adegoke OAJ
Journal of nutritional science 2024;13:e66

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Integrative proteogenomic profiling of high-risk prostate cancer samples from Chinese patients indicates metabolic vulnerabilities and diagnostic biomarkers.

Dong B, Xu JY, Huang Y, Guo J, Dong Q, Wang Y, Li N, Liu Q, Zhang M, Pan Q, Wang H, Jiang J, Chen B, Shen D, Ma Y, Zhai L, Zhang J, Li J, Xue W, Tan M, Qin J
Nature cancer 2024 Sep;5(9):1427-1447

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Branched-chain α-ketoacids aerobically activate HIF1α signalling in vascular cells.

Xiao W, Shrimali N, Vigder N, Oldham WM, Clish CB, He H, Wong SJ, Wertheim BM, Arons E, Haigis MC, Leopold JA, Loscalzo J
Nature metabolism 2024 Nov;6(11):2138-2156

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Notch1 blockade by a novel, selective anti-Notch1 neutralizing antibody improves immunotherapy efficacy in melanoma by promoting an inflamed TME.

de Freitas JT, Thakur V, LaPorte KM, Thakur VS, Flores B, Caicedo V, Ajaegbu CGE, Ingrasci G, Lipman ZM, Zhang K, Qiu H, Malek TR, Bedogni B
Journal of experimental & clinical cancer research : CR 2024 Nov 4;43(1):295

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A spatiotemporal proteomic map of human adipogenesis.

Klingelhuber F, Frendo-Cumbo S, Omar-Hmeadi M, Massier L, Kakimoto P, Taylor AJ, Couchet M, Ribicic S, Wabitsch M, Messias AC, Iuso A, Müller TD, Rydén M, Mejhert N, Krahmer N
Nature metabolism 2024 May;6(5):861-879

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Branched chain α-ketoacids aerobically activate HIF1α signaling in vascular cells.

Xiao W, Shrimali N, Oldham WM, Clish CB, He H, Wong SJ, Wertheim BM, Arons E, Haigis MC, Leopold JA, Loscalzo J
bioRxiv : the preprint server for biology 2024 May 30;

---

Maintenance of the branched-chain amino acid transporter LAT1 counteracts myotube atrophy following chemotherapy.

Mora S, Adegoke OAJ
American journal of physiology. Cell physiology 2024 Mar 1;326(3):C866-C879

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Interior decorative volatile organic compounds exposure induces sleep disorders through aberrant branched chain amino acid transaminase 2 mediated glutamatergic signaling resulting from a neuroinflammatory cascade.

Zheng J, Wu M, Pang Y, Liu Q, Liu Y, Jin X, Tang J, Bao L, Niu Y, Zheng Y, Zhang R
The Science of the total environment 2024 Jul 15;934:173254

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Branched-Chain Amino Acid Accumulation Fuels the Senescence-Associated Secretory Phenotype.

Liang Y, Pan C, Yin T, Wang L, Gao X, Wang E, Quang H, Huang D, Tan L, Xiang K, Wang Y, Alexander PB, Li QJ, Yao TP, Zhang Z, Wang XF
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2024 Jan;11(2):e2303489

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Branched-chain keto acids inhibit mitochondrial pyruvate carrier and suppress gluconeogenesis in hepatocytes.

Nishi K, Yoshii A, Abell L, Zhou B, Frausto R, Ritterhoff J, McMillen TS, Sweet I, Wang Y, Gao C, Tian R
Cell reports 2023 Jun 27;42(6):112641

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The duration of glucocorticoid treatment alters the anabolic response to high-force muscle contractions.

Dunlap KR, Steiner JL, Hickner RC, Chase PB, Gordon BS
Journal of applied physiology (Bethesda, Md. : 1985) 2023 Jul 1;135(1):183-195

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METTL16 drives leukemogenesis and leukemia stem cell self-renewal by reprogramming BCAA metabolism.

Han L, Dong L, Leung K, Zhao Z, Li Y, Gao L, Chen Z, Xue J, Qing Y, Li W, Pokharel SP, Gao M, Chen M, Shen C, Tan B, Small A, Wang K, Zhang Z, Qin X, Yang L, Wunderlich M, Zhang B, Mulloy JC, Marcucci G, Chen CW, Wei M, Su R, Chen J, Deng X
Cell stem cell 2023 Jan 5;30(1):52-68.e13

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Downregulation of extramitochondrial BCKDH and its uncoupling from AMP deaminase in type 2 diabetic OLETF rat hearts.

Ogawa T, Kouzu H, Osanami A, Tatekoshi Y, Sato T, Kuno A, Fujita Y, Ino S, Shimizu M, Toda Y, Ohwada W, Yano T, Tanno M, Miki T, Miura T
Physiological reports 2023 Feb;11(4):e15608

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Branched-chain amino acid catabolism breaks glutamine addiction to sustain hepatocellular carcinoma progression.

Yang D, Liu H, Cai Y, Lu K, Zhong X, Xing S, Song W, Zhang Y, Ye L, Zhu X, Wang T, Zhang P, Li ST, Feng J, Jia W, Zhang H, Gao P
Cell reports 2022 Nov 22;41(8):111691

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Ruscogenin Alleviates Myocardial Ischemia-Induced Ferroptosis through the Activation of BCAT1/BCAT2.

Fu F, Lai Q, Hu J, Zhang L, Zhu X, Kou J, Yu B, Li F
Antioxidants (Basel, Switzerland) 2022 Mar 18;11(3)

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Targeting BCAT1 Combined with α-Ketoglutarate Triggers Metabolic Synthetic Lethality in Glioblastoma.

Zhang B, Peng H, Zhou M, Bao L, Wang C, Cai F, Zhang H, Wang JE, Niu Y, Chen Y, Wang Y, Hatanpaa KJ, Copland JA, DeBerardinis RJ, Wang Y, Luo W
Cancer research 2022 Jul 5;82(13):2388-2402

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BCAA-BCKA axis regulates WAT browning through acetylation of PRDM16.

Ma QX, Zhu WY, Lu XC, Jiang D, Xu F, Li JT, Zhang L, Wu YL, Chen ZJ, Yin M, Huang HY, Lei QY
Nature metabolism 2022 Jan;4(1):106-122

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3,6-dichlorobenzo[b]thiophene-2-carboxylic acid alleviates ulcerative colitis by suppressing mammalian target of rapamycin complex 1 activation and regulating intestinal microbiota.

He QZ, Wei P, Zhang JZ, Liu TT, Shi KQ, Liu HH, Zhang JW, Liu SJ
World journal of gastroenterology 2022 Dec 14;28(46):6522-6536

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Pyridostigmine Protects Against Diabetic Cardiomyopathy by Regulating Vagal Activity, Gut Microbiota, and Branched-Chain Amino Acid Catabolism in Diabetic Mice.

Yang Y, Zhao M, He X, Wu Q, Li DL, Zang WJ
Frontiers in pharmacology 2021;12:647481

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Branched-chain amino acids govern the high learning ability phenotype in Tokai high avoider (THA) rats.

Shida Y, Endo H, Owada S, Inagaki Y, Sumiyoshi H, Kamiya A, Eto T, Tatemichi M
Scientific reports 2021 Nov 29;11(1):23104

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Regulation of branched-chain amino acid metabolism by hypoxia-inducible factor in glioblastoma.

Zhang B, Chen Y, Shi X, Zhou M, Bao L, Hatanpaa KJ, Patel T, DeBerardinis RJ, Wang Y, Luo W
Cellular and molecular life sciences : CMLS 2021 Jan;78(1):195-206

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Oxoeicosanoid receptor inhibition alleviates acute myocardial infarction through activation of BCAT1.

Lai Q, Yuan G, Shen L, Zhang L, Fu F, Liu Z, Zhang Y, Kou J, Liu S, Yu B, Li F
Basic research in cardiology 2021 Jan 23;116(1):3

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Transketolase Deficiency in Adipose Tissues Protects Mice From Diet-Induced Obesity by Promoting Lipolysis.

Tian N, Liu Q, Li Y, Tong L, Lu Y, Zhu Y, Zhang P, Chen H, Hu L, Meng J, Feng M, Li M, Zheng L, Li B, Xu T, Wu L, Tong X
Diabetes 2020 Jul;69(7):1355-1367

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BCAT2-mediated BCAA catabolism is critical for development of pancreatic ductal adenocarcinoma.

Li JT, Yin M, Wang D, Wang J, Lei MZ, Zhang Y, Liu Y, Zhang L, Zou SW, Hu LP, Zhang ZG, Wang YP, Wen WY, Lu HJ, Chen ZJ, Su D, Lei QY
Nature cell biology 2020 Feb;22(2):167-174

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Glucose promotes cell growth by suppressing branched-chain amino acid degradation.

Shao D, Villet O, Zhang Z, Choi SW, Yan J, Ritterhoff J, Gu H, Djukovic D, Christodoulou D, Kolwicz SC Jr, Raftery D, Tian R
Nature communications 2018 Jul 26;9(1):2935

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Metabolic effects of acute thiamine depletion are reversed by rapamycin in breast and leukemia cells.

Liu S, Miriyala S, Keaton MA, Jordan CT, Wiedl C, Clair DK, Moscow JA
PloS one 2014;9(1):e85702

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16417-1-AP(BCAT2 antibody) at dilution of 1:800

Sample type

tissue

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

The BCAT2 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human BCAT2. This antibody recognizes human,mouse,rat antigen. The BCAT2 antibody has been validated for the following applications: ELISA, IHC, WB analysis.

Appendix

16417-1-AP.pdf

Show more

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human skin using 16417-1-AP(BCAT2 antibody) at dilution of 1:50 (under 40x lens)

Sample type

tissue